010-00344R

X-Cite® 110LED User Guide X-Cite® 110LED User Guide 035-00575R rev. 1 Made in Canada Excelitas Canada Inc. 2015 All rights reserved No part of this publication may be reproduced, transmitted, transcribed, stored in a retrieval system or translated into any language in any form by any means without the prior written consent of Excelitas Canada Inc. Every effort has been made to ensure information in this manual is accurate; however, information in this manual is subject to change without notice and does not represent a commitment on the part of the authors. Excelitas Canada Inc. 2260 Argentia Road Mississauga ON L5N 6H7 CANADA Trademarks X-Cite® is a registered trademark of Excelitas Canada Inc. All rights reserved. All other product names are trademarks of their respective owners. 2 X-Cite® 110LED User Guide 035-00575R rev 1 Table of Contents 1 Introduction ...............................................................................................................................5 2 Safety.........................................................................................................................................6 2.1 2.2 3 Getting Started ...........................................................................................................................8 3.1 3.2 4 Glossary of Symbols ...................................................................................................................... 6 Safety Precautions ........................................................................................................................ 6 System Components ..................................................................................................................... 8 Installation/Set-up ........................................................................................................................ 9 Operation – Manual Control ..................................................................................................... 12 4.1 The Basics .................................................................................................................................... 12 4.2 SpeedDIAL Home Screen............................................................................................................. 12 4.3 SpeedDIAL Menu and Settings .................................................................................................... 13 4.3.1 SpeedDIAL Menu Structure................................................................................................. 13 4.3.2 LCD – Display Screen Brightness and Color Settings ........................................................... 14 4.3.3 Favo – Favorite Intensity Setting......................................................................................... 14 4.3.4 TTL –TTL Mode Control ....................................................................................................... 15 4.3.5 Hand – Display Screen Orientation ..................................................................................... 15 4.3.6 Srvc – Service Data .............................................................................................................. 15 5 Operation - External Control ..................................................................................................... 16 5.1 USB/RS-232 ................................................................................................................................. 16 5.1.1 Driver Installation (via internet).......................................................................................... 16 5.1.2 Driver Installation (via ZIP file) ............................................................................................ 17 5.1.3 Verify Installation & Get COM Port Number....................................................................... 17 5.1.4 X-Cite Control Panel / GUI installation (via ZIP file) ............................................................ 18 5.1.5 X-Cite Control Panel – Tips for Use ..................................................................................... 18 5.1.6 Commercial Software Support ............................................................................................ 20 5.1.7 Software Developer’s Kit (SDK) ........................................................................................... 20 5.2 TTL ............................................................................................................................................... 20 5.2.1 TTL Mode ............................................................................................................................ 20 5.2.2 TTL Mode Timeout .............................................................................................................. 20 5.2.3 TTL Timing Diagram............................................................................................................. 22 5.2.4 TTL Signal and LED Status.................................................................................................... 22 5.2.5 TTL Input Specifications ...................................................................................................... 22 6 Troubleshooting ....................................................................................................................... 23 6.1 6.2 6.3 6.4 7 Error Messages............................................................................................................................ 23 Failure to Power Up .................................................................................................................... 23 Low Illumination Intensity .......................................................................................................... 24 Other Potential Symptoms & Questions ..................................................................................... 24 Routine Care and Maintenance ................................................................................................. 25 7.1 7.2 7.3 General........................................................................................................................................ 25 Cleaning - Exterior Surfaces ........................................................................................................ 25 Cleaning - Optical Surfaces ......................................................................................................... 25 3 X-Cite® 110LED User Guide 035-00575R rev 1 8 Technical Specifications ............................................................................................................ 26 8.1 8.2 8.3 8.4 8.5 8.6 9 General........................................................................................................................................ 26 Electrical ...................................................................................................................................... 26 Environmental – Operating Conditions ...................................................................................... 27 Environmental – Transport and Storage Conditions................................................................... 27 Input/Output (I/O) Connections ................................................................................................. 27 Output Stability ........................................................................................................................... 27 Regulatory Compliance ............................................................................................................. 28 9.1 9.2 9.3 9.4 9.5 10 10.1 10.2 11 11.1 11.2 11.3 Product Safety and Electromagnetic Compatibility .................................................................... 28 CE Marking .................................................................................................................................. 28 FCC .............................................................................................................................................. 28 WEEE Directive ............................................................................................................................ 28 China RoHS .................................................................................................................................. 29 Warranty & Repairs .............................................................................................................. 30 Warranty Terms .......................................................................................................................... 30 Returning equipment to Excelitas............................................................................................... 30 Contact Information.............................................................................................................. 31 General........................................................................................................................................ 31 Technical Support and Service .................................................................................................... 31 Accessories and Replacement Parts ........................................................................................... 31 Table of Figures Figure 1 System Components ...................................................................................................................... 8 Figure 2 Front and Rear Panel Connections ................................................................................................. 9 Figure 3 SpeedDIAL ...................................................................................................................................... 9 Figure 4 Correct position for fully inserted X-Cite LLG and LLG with A and B insertion lines ..................... 11 Figure 5 SpeedDIAL Home Screen Icons .................................................................................................... 12 Figure 6 Device Manager, COM Port Listing ............................................................................................... 18 Figure 7 X-Cite Control Panel ..................................................................................................................... 19 Figure 8 Sequence of Events for TTL Mode Timeout (set to 4 hours) ........................................................ 21 4 X-Cite® 110LED User Guide 035-00575R rev 1 1 Introduction Meet X-Cite 110LED - a compact white light LED source for routine fluorescence applications. Using XCite’s traditional liquid light guides, X-Cite 110LED delivers superior optical power and exceptional field uniformity at the specimen level with the broadest spectrum of fluorescence excitation through manual, PC and TTL control. With LEDs rated to 25,000 hours and no lamps or modules to replace, X-Cite 110LED offers simplicity and convenience to researchers allowing them to focus on their experiments instead of equipment maintenance. This product is intended for use in fluorescence microscopy illumination. It allows researchers the ability to excite fluorescence in samples being studied and characterize their location or behaviour. Excelitas Technologies Corp. is a global technology leader focused on delivering innovative, customized solutions to meet the lighting, detection and other high-performance technology needs of customers. XCite, formerly of Lumen Dynamics (which was acquired by Excelitas Technologies Corp. in November 2013) offers the Life Science and Analytical Instrumentation market a broad range of innovative lamp and LED fluorescence illumination and measurement solutions. Recognized as the industry standard in research microscopy, X-Cite also provides solid state LED technologies to instrument manufacturers, and combines maximum optical performance with flexible design, as well as best-in-class manufacturing quality and lead time. Excelitas recommends reading this manual to discover all the features available in X-Cite 110LED. Thank you for choosing X-Cite! 5 X-Cite® 110LED User Guide 035-00575R rev 1 2 Safety 2.1 Glossary of Symbols Symbol Meaning CAUTION - Risk of danger: consult accompanying documents WARNING – Eye damage may result from directly viewing ultraviolet light. Protective eye shielding and clothing must be used at all times. WARNING – UV light hazard. Input/Output Signals Input Signal CAUTION – Hot surface 2.2 Safety Precautions Please observe the following safety precautions at all times during operation and maintenance of this product. Failure to do so may result in personal injury or property damage. 1. UV emitted from this product. Avoid eye and skin exposure to unshielded product. Do not look at operating lamp/LED. Eye injury may result. 2. Never look into the light emitting end of the light guide. The light could severely damage the cornea and retina of the eye if the light is observed directly. Eye shielding must be used at all times as well as clothing to protect exposed skin. 3. Always make sure the light guide and microscope adapter (if applicable) are securely attached to the microscope prior to turning on power to the unit. This will minimize the risk of exposure to the UV light. 4. Always use this product with the external power supply and power cord set provided. Ensure the external power supply cord is connected to a grounded 3-pin outlet only. Any substitution of these components will invalidate regulatory certification of this product and may impair operating safety. 6 X-Cite® 110LED User Guide 035-00575R rev 1 5. Disconnecting of main supply source is done by putting the on/off button in the off position and unplugging the power cord. 6. It is recommended that ONLY QUALIFIED TECHNICAL PERSONNEL perform any testing or repairs of this unit. If servicing the unit, disconnect the external power supply before opening the cover of this unit. All cover screws must be replaced prior to applying power to the unit, or safety of the unit will be impaired. Note that opening the cover and/or altering the unit in any way may void the warranty (see section 10 for more details). 7. Monitoring the unit during manual operation The level of UV and visible energy supplied by this product is sufficient to ignite flammable substances. During manual operation, the unit must be attended at all times by a qualified operator. The unit must not be left unattended while turned on. If an operator leaves the work area of the unit, the power switch must be turned off. 8. Monitoring the unit during automated operation The level of UV and visible energy supplied by this product is sufficient to ignite flammable substances. Therefore, when the unit is operated unattended in an automated environment, an alarm function must be provided by the user to indicate a malfunction in the associated equipment used. 9. Should this X-Cite unit be used in a manner not specified by Excelitas, the protection provided by the equipment may be impaired. 10. This unit is designed for bench top use only! Always ensure that the unit is placed on a hard, stable surface, ensuring that ventilation openings are not obstructed. Any obstruction of these openings could result in a possible over-heating condition. 11. Any electronic equipment connected to this product must comply with the requirements of EN/IEC 60950. 12. To clean the exterior of the unit, use a slightly dampened cloth and a simple water/ detergent solution only. Avoid the optical surfaces and lenses. Cleaning of optics should only be attempted by qualified personnel using appropriate fluids and lens paper. 7 X-Cite® 110LED User Guide 035-00575R rev 1 3 Getting Started 3.1 System Components This X-Cite system contains the following components: 1. 2. 3. 4. 5. X-Cite 110LED unit SpeedDIAL Manual Controller Power Supply and Power Cord Microscope Adaptor (if ordered) Accessories Box, containing: a. Quick Start Instruction Sheet (user guide, software and driver downloads: http://www.excelitas.com/Pages/Product/X-Cite-110LED.aspx) b. Liquid Light Guide c. USB Cable d. Hex tool, 3mm If any components are missing or appear damaged, please contact Excelitas immediately. Figure 1 System Components 8 X-Cite® 110LED User Guide 035-00575R rev 1 Figure 2 Front and Rear Panel Connections Figure 3 SpeedDIAL 3.2 Installation/Set-up 1. Unpack a. Carefully unpack the unit and accessories from the shipping carton. 9 X-Cite® 110LED User Guide 035-00575R rev 1 b. When unpacking the light guide (liquid or quartz fibre), take care not to bend sharply or kink. 2. Install the Microscope Adaptor (if applicable) a. Remove the protective wrap from the Microscope Adaptor, being careful not to touch the lens surface. b. Insert the flange portion of the Microscope Adaptor into the light port on the microscope, and secure it using the hardware provided on the microscope. (Refer to relevant microscope user manual for complete instructions on mounting a standard epifluorescence lamphouse.) General guidelines: i. Carl Zeiss – Tighten the hex fastener on the side of the light port with the 3mm Hex Tool . ii. Leica - Tighten the hex fastener on the side of the light port with the 3mm Hex Tool . iii. Nikon – Line up the “notch” with the pin on the microscope fitting, hold the adaptor firmly against the light port, and twist the collar to lock the components together. iv. Olympus - Tighten the hex fastener(s) with the 3mm Hex Tool. Depending on the model, there may be one (1) fastener on the side, or two (2) fasteners located at the 10:00 and 2:00 positions. 3. Install the Light Guide a. Remove the red plug from the light guide port located on the front panel. b. Remove the red caps from the ends of the light guide. c. Insert the larger end of the light guide into the light guide port. For X-Cite 110LED, the light guide must be inserted up to the “B” line printed on the grey ferrule, see Figure 4. [Note: If using an X-Cite 3mm or 5mm light guide with only one “insertion line” on the grey ferrule, this line will be 20mm away from the light guide port.] d. Insert the smaller end of the light guide into the Microscope Adaptor (or light guide port on the microscope/imaging instrument). Ensure that the light guide is fully inserted and lock it in place with the thumbscrew (or appropriate fastener). 10 X-Cite® 110LED User Guide 035-00575R rev 1 Figure 4 Correct position for fully inserted X-Cite LLG and LLG with A and B insertion lines 4. Check Position of the Equipment a. The X-Cite unit should be positioned to avoid sharp bends and strain on the light guide. Ideally, the unit should be positioned so that: i. The light guide has as few bends as possible. ii. Bends have the largest bend radius possible. iii. There is some slack in the light guide. b. All air vents on the side panel of the unit should be clear of obstruction. Recommended clearance is 20cm (8 inches). c. Note that the main power switch is located on the rear panel of the unit. The unit should be placed so that the switch is convenient for the operator to access. 5. Connect speedDIAL to main unit a. Important: Ensure that main unit is powered OFF before attempting to connect speedDIAL, or damage to the device may result. b. Insert the mini DIN plug into the “Remote” port on the rear of the unit. Ensure the arrow mark on the connector is on the right (i.e. at the 3:00 position). Note: Never force the connector – this can damage the pins. If connector does not insert smoothly, stop and check for bent pins. c. Place speedDIAL next to the microscope, or another easily accessible location. 6. Connect USB (if using) a. Insert “B” (square) end into the “USB” port on rear of the main unit. b. Insert “A” (flat) end into an available port on the computer. c. Note: For best performance, use the supplied USB cable or one of equivalent quality and length. Using a longer USB cable than the one supplied may result in intermittent communication errors. 11 X-Cite® 110LED User Guide 035-00575R rev 1 7. Connect AC Power a. Connect Power Supply: Insert the power supply’s 4-pin connector into the “Power” port on rear of the main unit. Ensure that the orientation is correct, or else damage to the pins may result. The arrows on the plug should be on the right (i.e. at the 3:00 position). b. Connect Power Cord: Connect female end of the power cord to AC port on the power supply. Connect male end to a properly grounded electrical outlet. c. For safe operation, use only the power cord supplied or one with an equivalent rating. 4 Operation – Manual Control 4.1 The Basics 1. Start the unit a. Flip the rocker switch on rear of the main unit to turn X-Cite system on. b. The system will have a brief initialization period (approximately 5 seconds). The speedDIAL display will show “X-Cite” during this time. c. When the display shows “x%”, it is ready to use. d. Note: If speedDIAL will be used for manual control, it must be connected before turning system on. SpeedDIAL can be damaged if it is plugged into, or unplugged from, the system while it is powered on. 2. Illuminate a specimen a. Click the dial (shutter button) on speedDIAL to turn on/off the excitation light. 3. Adjust intensity a. Turn the dial to adjust intensity - clockwise to increase, counter-clockwise to decrease. b. The dial is speed sensitive – turning slowly will allow adjustments in smaller increments, turning quickly will increase the step sizes. c. Intensity can be adjusted in 1% increments from 5 to 100%. 4.2 SpeedDIAL Home Screen Figure 5 SpeedDIAL Home Screen Icons 12 X-Cite® 110LED User Guide 035-00575R rev 1 4.3 SpeedDIAL Menu and Settings In addition to the intuitive intensity adjustment and illumination ON/OFF control, speedDIAL has several advanced settings and control options.     To access the main menu, press and hold the dial for one (1) second. To navigate the menus, turn the dial to scroll through the options. An arrowhead will indicate the currently selected menu option; click the dial to make a selection. To adjust settings, turn the dial. To exit the setting adjustment, click the dial. To exit menu system at any time, press and hold the dial for one (1) second. Note: Setting changes will take effect immediately after selection. However, for the first five (5) minutes, the new settings are stored in a temporary memory location. If the X-Cite unit is powered down during this time, settings will revert back to their previous values. To ensure that new settings will be remembered, wait at least five (5) minutes before powering down the unit. 4.3.1 SpeedDIAL Menu Structure LCD     Favo     TTL    Hand Srvc        Exit Dim Brig Color Exit Int Enbl Spd Exit Enbl Time Exit Hour SN Tmp1 Tmp2 S/W1 S/W2 Exit 13 X-Cite® 110LED User Guide 035-00575R rev 1 4.3.2 LCD – Display Screen Brightness and Color Settings In the LCD sub menu, the backlight on the display screen can be turned on/off, set to a different brightness level, or set to a different color. a. Select the “LCD” option from main menu. b. To adjust LCD display backlight time out: i. Select “Dim” and scroll through the time out options, which are: Off and 1 thru 999 seconds in 1 second increments. “Off” will turn off the backlight timer (i.e. backlight will always be on). ii. Click dial to return to “LCD” menu. c. To adjust LCD brightness: i. Select “Brig” and scroll through % brightness settings until the desired level is reached. To turn backlight off, set level to 0%. ii. Click dial to return to “LCD” menu. d. To change LCD color: i. Select “Color” and scroll up /down through the options until desired color is on LCD. ii. Click dial to return to “LCD” menu. e. Select “Exit” to go back to the main menu, or press and hold dial to return to the home screen. 4.3.3 Favo – Favorite Intensity Setting In the FAVO sub menu, a favorite, commonly used intensity setting can be saved. When this mode is enabled, the intensity level will instantly jump to this setting with a double-click of the dial. A second double-click will toggle back to the previous intensity level. a. Select “Favo” option from main menu. b. To set/change the favorite intensity: i. Select “Int” and scroll through % intensity settings until the desired level is reached. ii. Click dial to save selection and return to “Favo” menu. iii. TIP: Scrolling through % settings in this menu will not actually change the output in real time, even if LED is on. The favorite intensity should be determined before entering this menu. c. To enable/disable favorite intensity mode: i. Select “Enbl” and scroll up/down to the desired setting.  “On” will enable (i.e. double-click speedDIAL = Favorite setting)  “Off” will disable (i.e. double-click speedDIAL = do nothing) ii. Click dial to save selection and return to “Favo” menu. d. To adjust the delay between clicks in the “double-click”: i. Select “Spd” and scroll up/down to the desired delay setting. “1” will provide the shortest delay, “10” will be the longest. ii. Click dial to save selection and return to “Favo” menu. 14 X-Cite® 110LED User Guide 035-00575R rev 1 iii. TIP: If double-clicking results in the light being turned OFF instead of going to favorite intensity, increase the delay setting. iv. Note: Setting long delay times will result in a longer response time for regular ON/OFF control with speedDIAL (the system must wait to see if the single-click turns into a double-click). Response times for TTL or USB on/off control will not be affected. 4.3.4 TTL –TTL Mode Control In the TTL menu, TTL mode can be enabled for experiments that require rapid LED ON/OFF control. For further information on TTL mode, refer to the External Control, TTL section 5.2. NOTE: TTL mode MUST be enabled for the system to respond quickly to a TTL signal. a. Select “TTL” option from main menu. b. To enable/disable TTL mode: i. Select the “Enbl” menu item. ii. Scroll to the desired setting, “On” to enable TTL or “Off” to disable TTL. iii. Click dial to return to “TTL” menu. c. To define the TTL timeout setting: i. Select the “Time” menu item. ii. Scroll through the time out options, which are: Never and 4 to 24 hours in half hour increments. “Never” will turn off the timeout feature (i.e. the cooling system and driver will always be active when TTL mode is enabled). iii. Click dial to return to “TTL” menu. 4.3.5 Hand – Display Screen Orientation In this menu, the LCD orientation can be rotated 180°. This allows operators to use speedDIAL on the left or right side of the microscope without blocking the view of the display with their hand. a. Select “Hand” option from main menu. b. Turn dial to toggle between “Right Hand” and “Left Hand” operation. Note that the screen will flip while adjusting this setting. c. Click dial to return to the main menu. 4.3.6 Srvc – Service Data In the Service menu, information specific to each X-Cite unit can be found. This includes total hours accumulated on the LED, unit serial number, temperatures measured at specific locations in the unit, and embedded software versions for the main unit and speedDIAL. Excelitas Tech Support personnel may request some or all of this information during a support call. a. Select “Srvc” option from main menu. b. To obtain the LED “in use” hours: i. Select the “Hour” menu option. ii. LED “hours of use” will be shown in one (1) hour increments from 0 to 999 hours. Due to space limitations on the LCD, when 1000 hours are logged, the 15 X-Cite® 110LED User Guide 035-00575R rev 1 format will change to “1.0k hours”, and increments will increase in 100 hour increments (e.g. 1142 hours will display as “1.1k hours”). The precise hour-byhour data will continue to be available via USB communication. iii. Click dial to return to “Srvc” menu. c. To obtain the unit serial number: i. Select the “SN” menu option. ii. Serial number of the unit will be shown. iii. Click dial to return to “Srvc” menu. d. To obtain current system temperatures: i. Select “Tmp1” or “Tmp2” as required. ii. Temperature will be shown in degrees Celsius. iii. Click dial to return to “Srvc” menu. e. To obtain the embedded software version numbers: i. Select the “S/W1” (for main unit) or “S/W2” (for speedDIAL) menu items. ii. The software version numbers will be shown in the format X.X.X. iii. Click dial to return to “Srvc” menu. 5 Operation - External Control 5.1 USB/RS-232 The X-Cite unit is a USB device which operates at USB 2.0 speeds of 12Mb/s and does not use a USB to RS-232 adapter. However, the X-Cite will appear as a COM port in Device Manager, and is functional as a COM port for programming purposes. This is done for legacy program support and ease of integration. A virtual COM port driver must be installed to enable Windows communication via the USB port. For computers running Windows which are connected to the internet, the driver will install automatically. For manual installation, the driver is available as a software download from the Excelitas website. Note that administrator privileges may be required to install drivers on your computer, in which case you may need to contact your IT department for assistance. 5.1.1 Driver Installation (via internet) For these instructions: internet is required, Windows 7 prompts are listed a. b. c. d. e. Ensure X-Cite unit is powered off. Ensure X-Cite unit is connected to the computer with the USB cable. Ensure computer is connected to internet. Power on X-Cite unit. Driver installation will begin automatically. A dialogue box will confirm installation has started. i. If the dialogue box reports that Driver installation was not successful, click on Get Details. 16 X-Cite® 110LED User Guide 035-00575R rev 1 f. ii. Click on option to Change Settings to Automatically search Windows for drivers. Confirm Yes, do this automatically, and save changes. A second dialogue box will confirm successful installation and provide a COM port number. Note the COM port number for use in other software applications. 5.1.2 Driver Installation (via ZIP file) For these instructions, download the driver ZIP file from the Excelitas website: http://www.excelitas.com/Pages/Product/X-Cite-110LED.aspx a. Ensure X-Cite unit is powered off. b. Ensure X-Cite unit is connected to the computer with the USB cable. c. Prepare the driver files by extracting and saving the files into a folder on desktop (or another easily accessible location). d. Power on X-Cite unit. e. The “new hardware found” wizard will appear. Select No to search Windows update for the software. Click Next to continue. f. Select Install from a list or specific location (Advanced) to locate the driver and click Next. Select Include this location in the search: and browse to the location on your hard drive where unzipped files were saved in step c. Click Next. g. Wait for installation to complete. Click Finish. 5.1.3 Verify Installation & Get COM Port Number a. Open the “Device Manager” utility on the computer. b. “X-Cite LED (XT600 Series)” will be listed under “Ports (COM & LPT)”. c. Note the COM port number for use in other software applications. 17 X-Cite® 110LED User Guide 035-00575R rev 1 Figure 6 Device Manager, COM Port Listing 5.1.4 X-Cite Control Panel / GUI installation (via ZIP file) For these instructions: ZIP file is available as a software download from the Excelitas website. a. Uninstall any previous versions of X-Cite Control Panel/GUI. (Previous versions released under the EXFO name will not be automatically replaced by versions 1.1.0 or later, and may cause confusion.) b. Prepare the driver files (if not already done in previous section) by extracting and saving the files into a folder on desktop (or another easily accessible location). c. To begin installation, go to unzipped files from step b, double-click on setup.exe or setup d. Setup Wizard will open. Follow prompts and click Close when Wizard is finished. e. To find GUI, go to: Start, All Programs, Excelitas Technologies, X-Cite Control Panel. Click to start/open GUI, or click-and-drag to copy a shortcut to desktop or Quick Launch toolbar. 5.1.5 X-Cite Control Panel – Tips for Use a. X-Cite Control Panel is available as a download from the X-Cite website. This tool provides an interface for controlling the X-Cite unit via PC and general testing of the communication port. 18 X-Cite® 110LED User Guide 035-00575R rev 1 b. Note that X-Cite Control Panel is designed to function with multiple X-Cite devices, and not all icons and features can be used with all devices. See the icon table below to determine which icons are relevant to control of this X-Cite unit. c. Icon color code: i. Green icons mean status is OK and/or the function is engaged ii. Yellow icons mean a warning or “getting ready” iii. Red icons mean there is an alarm condition iv. Grey icons mean a feature is not engaged, and/or not available v. If icons are ALL grey, X-Cite unit is not connected and/or turned off Figure 7 X-Cite Control Panel ICON NAME Alarm Temperature Calibration LED Hours Light Guide DESCRIPTION Green = All OK Red = Alarm on Click will clear alarm, unless unsafe to ignore Indicates system temperature status Yellow = warning, LED temp above normal Red = LED is close to (or has already exceeded) maximum safe temperature and may be turned off Not applicable for this X-Cite unit Indicates hours logged on LED Green = 20K hours, red = >40K hours Green = Light guide is inserted Red = Light guide is missing or not fully inserted Shutter Click to turn LED on/off Intensity Left mouse click = increase by 1% increment. Right mouse click = decrease by 1% increment. Left double click = opens dialog box, type in value 19 X-Cite® 110LED User Guide 035-00575R rev 1 Closed-Loop Feedback Not applicable for this X-Cite unit Lock/unlock Lock/unlock speedDIAL control Green = speedDIAL is locked Grey = speedDIAL is unlocked Lamp Not applicable for this X-Cite unit 5.1.6 Commercial Software Support a. This X-Cite unit can be controlled through many commercially available packages. Where model-specific control may not be available, basic functionality can be achieved using X-Cite 120PC, X-Cite exacte, or X-Cite 120LED hardware drivers. For an updated list of software packages that support X-Cite products, see http://www.excelitas.com/Downloads/XCite-softwareSupport.pdf b. For commercial software packages, if requested specify the serial port parameters as: 19,200 baud, no parity, 8 data bits and 1 stop bit. c. When controlling this X-Cite unit, some commercial software packages may block manual input from speedDIAL. The lock icon will appear on the speedDIAL display in these cases. 5.1.7 Software Developer’s Kit (SDK) The command list for this X-Cite unit is the same as for X-Cite 120LED, and is available by request. To obtain the latest update, please contact Technical Support. 5.2 TTL For high speed LED on/off control, TTL triggering can be used. The key thing to note about TTL control in this X-Cite unit is: TTL mode MUST be enabled for the system to respond quickly to a TTL signal. 5.2.1 TTL Mode TTL mode ensures that X-Cite’s cooling system and LED drivers are active between exposures, ensuring the fastest possible response to a TTL signal.  Enabling/disabling TTL mode can be accomplished manually via speedDIAL (refer to section 4.3.4) or via computer commands (refer to SDK).  ON/OFF status of the system will be updated on speedDIAL when it is controlled via TTL. However, due to the communication lag time between the main unit and speedDIAL, if LED “on time” is less than 30ms, the change may not be displayed. 5.2.2 TTL Mode Timeout There is a potential disadvantage of TTL mode - in this “always active” state, the system continues to consume energy and mechanical components accumulate wear. To help minimize unnecessary consumption at the end of an imaging session (or if equipment is accidentally left 20 X-Cite® 110LED User Guide 035-00575R rev 1 on), this unit is equipped with a TTL timeout setting. The factory default for the TTL timeout setting is “Never”, but defining a timeout is recommended for any imaging session that will end without an operator present to shut down equipment (e.g. middle of the night). Figure 8 Sequence of Events for TTL Mode Timeout (set to 4 hours) 21 X-Cite® 110LED User Guide 035-00575R rev 1 5.2.3 TTL Timing Diagram This plot shows typical TTL timing values when the unit is operated in TTL mode. These values should be used as a guide only. Actual values will be dependent on the LED intensity setting and the specific configuration/control hardware being used. Interval T1 Description Delay time, TTL trigger on to LED on Time (µs)