X-Cite® 120LEDmini User Guide
X-Cite® 120LEDmini User Guide
035-00587R rev. 1
Made in Canada
Excelitas Canada Inc. 2015
All rights reserved
No part of this publication may be reproduced, transmitted, transcribed, stored in a retrieval system or
translated into any language in any form by any means without the prior written consent of Excelitas
Canada Inc. Every effort has been made to ensure information in this manual is accurate; however,
information in this manual is subject to change without notice and does not represent a commitment on
the part of the authors.
Excelitas Canada Inc.
2260 Argentia Road
Mississauga ON L5N 6H7
CANADA
Trademarks
X-Cite® is a registered trademark of Excelitas Canada Inc. All rights reserved.
All other product names are trademarks of their respective owners.
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Table of Contents
1
Introduction ...............................................................................................................................5
2
Safety.........................................................................................................................................6
2.1
2.2
3
Getting Started ...........................................................................................................................8
3.1
3.2
4
Glossary of Symbols ...................................................................................................................... 6
Safety Precautions ........................................................................................................................ 6
System Components ..................................................................................................................... 8
Installation/Set-up ........................................................................................................................ 9
Operation – Manual Control ..................................................................................................... 12
4.1
The Basics .................................................................................................................................... 12
4.2
SpeedDIAL Home Screen............................................................................................................. 12
4.3
SpeedDIAL Menu and Settings .................................................................................................... 12
4.3.1
SpeedDIAL Menu Structure................................................................................................. 13
4.3.2
LCD – Display Screen Brightness and Color Settings ........................................................... 13
4.3.3
Favo – Favorite Intensity Setting......................................................................................... 14
4.3.4
TTL –TTL Mode Control ....................................................................................................... 14
4.3.5
Hand – Display Screen Orientation ..................................................................................... 15
4.3.6
Srvc – Service Data .............................................................................................................. 15
5
Operation - External Control ..................................................................................................... 16
5.1
USB/RS-232 ................................................................................................................................. 16
5.1.1
Driver Installation (via internet).......................................................................................... 16
5.1.2
Driver Installation (via ZIP file) ............................................................................................ 16
5.1.3
Verify Installation & Get COM Port Number....................................................................... 17
5.1.4
X-Cite® Control Panel / GUI installation .............................................................................. 17
5.1.5
X-Cite Control Panel – Tips for Use ..................................................................................... 18
5.1.6
Commercial Software Support ............................................................................................ 19
5.1.7
Software Developer’s Kit (SDK) ........................................................................................... 19
5.2
TTL ............................................................................................................................................... 20
5.2.1
TTL Mode ............................................................................................................................ 20
5.2.2
TTL Mode Timeout .............................................................................................................. 20
5.2.3
TTL Timing Diagram............................................................................................................. 21
5.2.4
TTL Signal and LED Status.................................................................................................... 21
5.2.5
TTL Input Specifications ...................................................................................................... 21
5.3
Foot Pedal Control (Optional) ..................................................................................................... 21
6
Troubleshooting ....................................................................................................................... 22
6.1
6.2
6.3
6.4
7
Error Messages............................................................................................................................ 22
Failure to Power Up .................................................................................................................... 22
Low Illumination Intensity .......................................................................................................... 23
Other Potential Symptoms & Questions ..................................................................................... 24
Routine Care and Maintenance ................................................................................................. 24
7.1
General........................................................................................................................................ 24
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7.2
7.3
7.4
8
Cleaning - Exterior Surfaces ........................................................................................................ 25
Cleaning - Optical Surfaces ......................................................................................................... 25
Thermal Management System .................................................................................................... 25
Technical Specifications ............................................................................................................ 26
8.1
8.2
8.3
8.4
8.5
8.6
9
General........................................................................................................................................ 26
Electrical ...................................................................................................................................... 26
Environmental – Operating Conditions ...................................................................................... 26
Environmental – Transport and Storage Conditions................................................................... 27
Input/Output (I/O) Connections ................................................................................................. 27
Output Stability ........................................................................................................................... 27
Regulatory................................................................................................................................ 27
9.1
9.2
9.3
9.4
9.5
10
10.1
10.2
11
11.1
11.2
Product Safety and Electromagnetic Compatibility .................................................................... 27
CE Marking .................................................................................................................................. 28
FCC .............................................................................................................................................. 28
WEEE Directive ............................................................................................................................ 28
China RoHS .................................................................................................................................. 29
Warranty & Repairs .............................................................................................................. 30
Warranty Terms .......................................................................................................................... 30
Returning equipment to Excelitas Technologies......................................................................... 30
Contact Information.............................................................................................................. 31
General........................................................................................................................................ 31
Accessories and Replacement Parts ........................................................................................... 31
Table of Figures
Figure 1 miniCUBE Front and Rear Panels ................................................................................................... 8
Figure 2 LED Head ........................................................................................................................................ 8
Figure 3 SpeedDIAL ...................................................................................................................................... 9
Figure 4 Installing Microscope Flange on LED Head .................................................................................. 10
Figure 5 SpeedDIAL Home Screen Icons .................................................................................................... 12
Figure 6 Device Manager, COM Port Listing ............................................................................................... 17
Figure 7 X-Cite® Control Panel .................................................................................................................... 18
Figure 8 Sequence of Events for TTL Mode Timeout (set to 4 hours) ........................................................ 20
Figure 9 Location and Removal of Fuse Tray in AC Receptacle .................................................................. 23
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1 Introduction
The new X-Cite® 120LEDmini provides LED illumination with no compromise. X-Cite® 120LEDmini
provides superior optical power and exceptional field uniformity at the specimen level with the broadest
spectrum of fluorescence excitation through manual, PC and TTL control. With LEDs rated to 20,000
hours and no lamps or modules to replace, X-Cite® 120LEDmini offers sheer simplicity in its compact size
and convenience to researchers allowing them to focus on their experiments instead of equipment
maintenance.
This product is intended for use in fluorescence microscopy illumination. It allows researchers the ability
to excite fluorescence in samples being studied and characterize their location or behavior.
Excelitas Technologies Corp. is a global technology leader focused on delivering innovative, customized
solutions to meet the lighting, detection and other high-performance technology needs of customers. XCite®, formerly of Lumen Dynamics (which was acquired by Excelitas Technologies Corp. in November
2013) offers the Life Science and Analytical Instrumentation market a broad range of innovative lamp
and LED fluorescence illumination and measurement solutions.
Excelitas Technologies recommends reading this manual to discover all the features available in X-Cite®
120LEDmini.
Thank you for choosing X-Cite®!
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2 Safety
2.1 Glossary of Symbols
Symbol
Meaning
CAUTION - Risk of danger: consult accompanying documents
WARNING – Eye damage may result from directly viewing ultraviolet light. Protective eye
shielding and clothing must be used at all times.
Input/Output Signals
Input Signal
CAUTION – Hot surface
2.2 Safety Precautions
Please observe the following safety precautions at all times during operation and maintenance of this
product. Failure to do so may result in personal injury or property damage.
1. UV emitted from this product. Avoid eye and skin exposure to unshielded product. Do not look
at operating lamp/LED. Eye injury may result.
2. Never look into the light emitting end of the LED head. The light could severely damage the
cornea and retina of the eye if the light is observed directly. Eye shielding must be used at all
times as well as clothing to protect exposed skin.
3. Always make sure the LED head is securely attached to the microscope prior to turning on
power to the unit. This will minimize the risk of exposure to the UV light.
4. To reduce the risk of fire or shock, always replace the fuses with the same type and rating.
5. Disconnecting of main supply source is done by putting the on/off button in the off “O” position
and unplugging the power cord.
6. It is recommended that ONLY QUALIFIED TECHNICAL PERSONNEL perform any testing or repairs
described in this manual. Disconnect the AC power cord from the unit before opening the cover
of this unit. All cover screws must be replaced prior to applying power to the unit, or safety of
the unit will be impaired.
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7. Monitoring the unit during manual operation
The level of UV and visible energy supplied by this product is sufficient to ignite flammable
substances. During manual operation, the unit must be attended at all times by a qualified
operator. The unit must not be left unattended while turned on. If an operator leaves the work
area of the unit, the power switch must be turned off.
8. Monitoring the unit during automated operation
The level of UV and visible energy supplied by this product is sufficient to ignite flammable
substances. Therefore, when the unit is operated unattended in an automated environment, an
alarm function must be provided by the user to indicate a malfunction in the associated
equipment used.
9. Should this X-Cite® unit be used in a manner not specified by Excelitas Technologies, the
protection provided by the equipment may be impaired.
10. This unit is designed for bench top use only! Always ensure that the unit is placed on a hard,
stable surface, ensuring that ventilation openings are not obstructed. Any obstruction of these
openings could result in a possible over-heating condition.
11. Any electronic equipment connected to this product must comply with the requirements of
EN/IEC 60950.
12. To clean the exterior of the unit, use a slightly dampened cloth and a simple water/ detergent
solution only. Avoid the optical surfaces and lenses. Cleaning of optics should only be attempted
by qualified personnel using appropriate fluids and lens paper.
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3 Getting Started
3.1 System Components
The X-Cite® 120LEDmini system contains the following components:
1.
2.
3.
4.
LED Head and miniCUBE (permanently connected via 1.5m cable)
SpeedDIAL Manual Controller
Microscope Flange
Accessories Box, containing:
a. Quick Start Instruction Sheet (User Guide, Software and Driver downloads are available
on: http://www.excelitas.com/Pages/Product/X-Cite-120LEDmini.aspx)
b. Hex Tool, 3mm
c. USB Cable
d. Power Cord
If any components are missing or appear damaged, please contact Excelitas Technologies immediately.
Connection to LED Head
AC in /
Fuse Tray
Main Power
ON/OFF
speedDIAL
IN/OUT USB IN/OUT
TTL IN
Foot Pedal IN
Figure 1 miniCUBE Front and Rear Panels
Side Vent
Microscope Flange
Figure 2 LED Head
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Rotating Dial
Display Screen (LCD)
Figure 3 SpeedDIAL
3.2 Installation/Set-up
1. Unpack
a. Carefully unpack the unit and accessories from the shipping carton.
b. When removing the LED Head and miniCUBE from the carton, ensure that both
components are supported and there is always some slack in the cable. Excessive strain
on the cable may damage or weaken the connection.
c. Do not use the cable as a “handle”.
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2. Install the Microscope Flange
a. Remove protective wrap from the Microscope Flange, being careful not to touch the
lens surfaces.
b. Remove protective cap from LED Head. Never power up the unit with this cap installed
– it can melt very quickly at high power settings and damage the unit.
c. Align the mounting holes on the Microscope Flange and the LED Head. Attach the
Microscope Flange using the Hex Tool and three (3) fasteners provided. The two (2)
dowel pins on the LED Head will allow you to install the Flange quickly and in the proper
position as required by your microscope.
Dowel Pins
Microscope Flange
Hex Fasteners (3)
Figure 4 Installing Microscope Flange on LED Head
3. Position the equipment
a. miniCUBE should be positioned to avoid sharp bends and strain on the cable.
b. The front panel of miniCUBE should be within reach to access the main power switch.
(During operation, all other functions are controlled via speedDIAL or USB interface.)
c. Air vents on both sides of miniCUBE should be clear of obstruction. Recommended
clearance is 20cm (8 inches) on all sides.
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4. Connect LED Head to Microscope
a. Insert Microscope Flange portion of the LED Head into the light port on the microscope,
and secure it using the hardware provided on the microscope. (Refer to relevant
microscope user manual for complete instructions on mounting a standard epifluorescence lamphouse.) General guidelines:
i. Carl Zeiss – Tighten the hex fastener on the side of the light port with the 3mm
Hex Tool.
ii. Leica - Tighten the hex fastener on the side of the light port with the 3mm Hex
Tool .
iii. Nikon – Line up the Microscope Flange “notch” with the pin on the microscope
fitting, hold the LED head firmly against the light port, and twist the collar to
lock the components together.
iv. Olympus - Tighten the hex fastener(s) with the 3mm Hex Tool. Depending on
the model, there may be one (1) fastener on the side, or two (2) fasteners
located at the 10:00 and 2:00 positions. Note for IX3-RFAL use: the epi-light
train aperture MUST be properly aligned before turning on X-Cite®
120LEDmini, or heat damage may result - see procedure in Olympus manual for
assistance.
b. Verify that the LED Head is oriented with the arrow label (rear side of the LED Head) is
pointing up.
c. Verify that the LED Head is securely fastened by attempting to gently rock it from side to
side.
5. Connect speedDIAL to miniCUBE
a. Insert the mini DIN plug into the “Remote” port on the rear of the miniCUBE. Ensure the
arrow mark on the connector is on top and centered. Note: Never force the connector
– this can damage the pins. If connector does not insert smoothly, stop and check for
bent pins.
b. Place speedDIAL next to the microscope, or another easily accessible location.
6. Connect USB (if using)
a. Insert “B” (square) end into the “USB” port on rear of the miniCUBE.
b. Insert “A” (flat) end into an available port on the computer.
c. Note: For best performance, use the supplied USB cable or one of equivalent quality
and length. Using a longer USB cable than the one supplied may result in intermittent
communication errors.
7. Connect AC Power
a. Connect female end to AC port on rear of the miniCUBE.
b. Connect male end to a properly grounded electrical outlet.
c. For safe operation, use only the power cord supplied or one with an equivalent rating.
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4 Operation – Manual Control
4.1 The Basics
1. Start the unit
a. Flip the rocker switch on the front of miniCUBE to turn X-Cite® 120LEDmini on.
b. The system will have a brief initialization period (approximately 10 seconds). The
speedDIAL display will show “X-Cite” during this time.
c. When the display shows “x%”, it is ready to use.
d. Note: If speedDIAL will be used for manual control, it must be connected before turning
system on. SpeedDIAL can be damaged if it is plugged into, or unplugged from, the
system while it is powered on.
2. Illuminate a specimen
a. Click the dial (shutter button) on speedDIAL to turn on/off the excitation light.
3. Adjust intensity
a. Turn the dial to adjust intensity - clockwise to increase, counter-clockwise to decrease.
b. The dial is speed sensitive – turning slowly will allow adjustments in smaller increments,
turning quickly will increase the step sizes.
4.2 SpeedDIAL Home Screen
LED Power Level
speedDIAL Locked
(via Computer)
LED ON
TTL Mode
Enabled
Figure 5 SpeedDIAL Home Screen Icons
4.3 SpeedDIAL Menu and Settings
In addition to the intuitive intensity adjustment and illumination ON/OFF control, speedDIAL has several
advanced settings and control options.
To access the main menu, press and hold the dial for one (1) second.
To navigate the menus, turn the dial to scroll through the options. An arrowhead will indicate
the currently selected menu option; click the dial to make a selection.
To adjust settings, turn the dial. To exit the setting adjustment, click the dial.
To exit menu system at any time, press and hold the dial for one (1) second.
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Note: Setting changes will take effect immediately after selection. However, for the first five (5)
minutes, the new settings are stored in a temporary memory location. If X-Cite® 120LEDmini is powered
down during this time, settings will revert back to their previous values. To ensure that new settings will
be remembered, wait at least five (5) minutes before powering down the unit.
4.3.1 SpeedDIAL Menu Structure
LCD
Favo
TTL
Hand
Srvc
Exit
Dim
Brig
Color
Exit
Int
Enbl
Spd
Exit
Enbl
Time
Exit
Hour
SN
Tmp1
Tmp2
S/W1
S/W2
Exit
4.3.2 LCD – Display Screen Brightness and Color Settings
In the LCD sub menu, the backlight on the display screen can be turned on/off, set to a different
brightness level, or set to a different color.
a. Select the “LCD” option from main menu.
b. To adjust LCD display backlight time out:
i. Select “Dim” and scroll through the time out options, which are: Off and 1 thru
999 seconds in 1 second increments. “Off” will turn off the backlight timer (i.e.
backlight will always be on).
ii. Click dial to save selection and return to “LCD” menu.
c. To adjust LCD brightness:
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i. Select “Brig” and scroll through % brightness settings until the desired level is
reached. To turn backlight off, set level to 0%.
ii. Click dial to save selection and return to “LCD” menu.
d. To change LCD color:
i. Select “Color” and scroll up /down through the options until desired color is on
LCD.
ii. Click dial to save selection and return to “LCD” menu.
e. Select “Exit” to go back to the main menu, or press and hold dial to return to the home
screen.
4.3.3 Favo – Favorite Intensity Setting
In the FAVO sub menu, a favorite, commonly used intensity setting can be saved. When this
mode is enabled, the intensity level will instantly jump to this setting with a double-click of the
dial. A second double-click will toggle back to the previous intensity level.
a. Select “Favo” option from main menu.
b. To set/change the favorite intensity:
i. Select “Int” and scroll through % intensity settings until the desired level is
reached.
ii. Click dial to save selection and return to “Favo” menu.
iii. TIP: Scrolling through % settings in this menu will not actually change the output
in real time, even if LED is on. The favorite intensity should be determined
before entering this menu.
c. To enable/disable favorite intensity mode:
i. Select “Enbl” and scroll up/down to the desired setting.
“On” will enable (i.e. double-click speedDIAL = Favorite setting)
“Off” will disable (i.e. double-click speedDIAL = do nothing)
ii. Click dial to save selection and return to “Favo” menu.
d. To adjust the delay between clicks in the “double-click”:
i. Select “Spd” and scroll up/down to the desired delay setting. “1” will provide
the shortest delay, “10” will be the longest.
ii. Click dial to save selection and return to “Favo” menu.
iii. TIP: If double-clicking results in the light being turned OFF instead of going to
favorite intensity, increase the delay setting.
iv. Note: Setting long delay times will result in a longer response time for regular
ON/OFF control with speedDIAL (the system must wait to see if the single-click
turns into a double-click). Response times for TTL, USB or foot pedal on/off
control will not be affected.
4.3.4 TTL –TTL Mode Control
In the TTL menu, TTL mode can be enabled for experiments that require rapid LED ON/OFF
control. For further information on TTL mode, refer to the External Control, TTL section 5.2.
NOTE: TTL mode MUST be enabled for the system to respond to a TTL signal.
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a. Select “TTL” option from main menu.
b. To enable/disable TTL mode:
i. Select the “Enbl” menu item.
ii. Scroll to the desired setting, “On” to enable TTL or “Off” to disable TTL.
iii. Click dial to save selection and return to “TTL” menu.
c. To define the TTL timeout setting:
i. Select the “Time” menu item.
ii. Scroll through the time out options, which are: Never and 4 to 24 hours in half
hour increments. “Never” will turn off the timeout feature (i.e. the cooling
system and driver will always be active when TTL mode is enabled).
iii. Click dial to save selection and return to “TTL” menu.
4.3.5 Hand – Display Screen Orientation
In this menu, the LCD orientation can be rotated 180°. This allows operators to use speedDIAL
on the left or right side of the microscope without blocking the view of the display with their
hand.
a. Select “Hand” option from main menu.
b. Turn dial to toggle between “Right Hand” and “Left Hand” operation. Note that the
screen will flip while adjusting this setting.
c. Click dial to save selection and return to the main menu.
4.3.6 Srvc – Service Data
In the Service menu, information specific to each X-Cite® 120LEDmini unit can be found. This
includes total hours accumulated on the LED, unit serial number, temperatures measured at
specific locations in the unit, and embedded software versions for miniCUBE and speedDIAL.
Excelitas Tech Support personnel may request some or all of this information during a support
call.
a. Select “Srvc” option from main menu.
b. To obtain the LED “in use” hours:
i. Select the “Hour” menu option.
ii. LED “hours of use” will be shown in one (1) hour increments from 0 to 999
hours. Due to space limitations on the LCD, when 1000 hours are logged, the
format will change to “1.0k hours”, and increments will increase to 100 hours
(e.g. 1142 hours will display as “1.1k hours”). The precise hour-by-hour data will
continue to be available via USB communication.
iii. Click dial to return to “Srvc” menu.
c. To obtain the unit serial number:
i. Select the “SN” menu option.
ii. Serial number of the unit will be shown.
iii. Click dial to return to “Srvc” menu.
d. To obtain current system temperatures:
i. Select “Tmp1” or “Tmp2” as required.
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ii. Temperature will be shown in degrees Celsius.
iii. Click dial to return to “Srvc” menu.
e. To obtain the embedded software version numbers:
i. Select the “S/W1” or “S/W2” menu items.
ii. The software version numbers will be shown in the format X.X.X.
iii. Click dial to return to “Srvc” menu.
5 Operation - External Control
5.1 USB/RS-232
A virtual COM port driver must be installed to enable Windows communication via the USB port. For
computers running Windows which are connected to the internet, the driver will install automatically.
For manual installation, the driver is available as a software download from the Excelitas website. Note
that administrator privileges may be required to install drivers on your computer, in which case you may
need to contact your IT department for assistance.
5.1.1 Driver Installation (via internet)
For these instructions: internet is required, Windows 7 prompts are listed
a.
b.
c.
d.
e.
f.
Ensure X-Cite® 120LEDmini is powered off.
Ensure X-Cite® 120LEDmini is connected to the computer with the USB cable.
Ensure computer is connected to internet.
Power on X-Cite® 120LEDmini.
Driver installation will begin automatically. A dialogue box will confirm installation has
started.
i. If the dialogue box reports that Driver installation was not successful, click on
Get Details.
ii. Click on option to Change Settings to Automatically search Windows for
drivers. Confirm Yes, do this automatically, and save changes.
A second dialogue box will confirm successful installation and provide a COM port
number. Note the COM port number for use in other software applications.
5.1.2 Driver Installation (via ZIP file)
For these instructions, download the driver ZIP file from the Excelitas website:
http://www.excelitas.com/Pages/Product/X-Cite-120LEDmini.aspx
a. Ensure X-Cite® 120LEDmini is powered off.
b. Ensure X-Cite® 120LEDmini is connected to the computer with the USB cable.
c. Prepare the driver files by extracting and saving the files into a folder on the desktop (or
another easily accessible location).
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d. Power on X-Cite® 120LEDmini.
e. The “new hardware found” wizard will appear. Select No to search Windows update for
the software. Click Next to continue.
f. Select Install from a list or specific location (Advanced) to locate the driver and click
Next. Select Include this location in the search and browse to the location on your hard
drive where unzipped files were saved in step c. Click Next.
g. Wait for installation to complete. Click Finish.
5.1.3 Verify Installation & Get COM Port Number
a. Open the “Device Manager” utility on the computer.
b. “X-Cite 120LED USB Communications” will be listed under “Ports (COM & LPT)”.
c. Note the COM port number for use in other software applications.
Figure 6 Device Manager, COM Port Listing
5.1.4 X-Cite® Control Panel / GUI installation
For these instructions: ZIP file is available as a software download from the Excelitas website.
a. Uninstall any previous versions of X-Cite® Control Panel/GUI. (Previous versions
released under the EXFO name will not be automatically replaced by versions 1.1.0 or
later, and may cause confusion.)
b. Prepare the driver files (if not already done in previous section) by extracting and saving
the files into a folder on desktop (or another easily accessible location).
c. To begin installation, go to unzipped files from step b, double-click on setup.exe or
setup
d. Setup Wizard will open. Follow prompts and click Close when Wizard is finished.
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e. To find GUI, go to: Start, All Programs, Excelitas Technologies, X-Cite Control Panel.
Click to start/open GUI, or click-and-drag to copy a shortcut to desktop or Quick Launch
toolbar.
5.1.5 X-Cite Control Panel – Tips for Use
a. X-Cite Control Panel is available as a download from the Excelitas website. This tool
provides an interface for controlling the X-Cite® 120LEDmini via PC and general testing
of the communication port.
b. Note that X-Cite® Control Panel is designed to function with multiple X-Cite® devices,
and not all icons and features can be used with all devices. See the icon table below to
determine which icons are relevant to control of X-Cite® 120LEDmini.
c. Icon color code:
i. Green icons mean status is OK and/or the function is engaged
ii. Yellow icons mean a warning or “getting ready”
iii. Red icons mean there is an alarm condition
iv. Grey icons mean a feature is not engaged, and/or not available
v. If icons are ALL grey, X-Cite® 120LEDmini is not connected and/or turned off
Hide/Unhide
Controls
Control Icons
Power Level
LED Hours of Use
Figure 7 X-Cite Control Panel
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ICON
NAME
Alarm
Temperature
DESCRIPTION
Green = All OK
Red = Alarm on
Click will clear alarm, unless unsafe to ignore
Indicates system temperature status
Yellow = warning, LED temp above normal
Red = LED off, temp is too high
Calibration
Not applicable for X-Cite® 120LEDmini
LED Hours
Indicates hours logged on LED
Green = 20K hours, red = >40K hours
Light Guide
Not applicable for X-Cite® 120LEDmini
Shutter
Click to turn LED on/off
Intensity
Left mouse click = increase by 1% increment.
Right mouse click = decrease by 1% increment.
Left double click = opens dialog box type in value
Closed-Loop
Feedback
Not applicable for X-Cite® 120LEDmini
Lock/unlock
Lock/unlock speedDIAL control
Green = speedDIAL is locked
Grey = speedDIAL is unlocked
Lamp
Not applicable for X-Cite® 120LEDmini
5.1.6 Commercial Software Support
a. X-Cite® 120LEDmini can be controlled through many commercially available packages.
Where X-Cite® 120LEDmini specific control may not be available, basic functionality can
be achieved using X-Cite® 120PC or X-Cite® exacte hardware drivers. For an updated list
of software packages that support X-Cite® products, see
http://www.excelitas.com/Downloads/XCite-softwareSupport.pdf
b. For commercial software packages, if requested specify the serial port parameters as:
19,200 baud, no parity, 8 data bits and 1 stop bit.
c. When controlling X-Cite® 120LEDmini, some commercial software packages may block
manual input from speedDIAL. The lock icon will appear on the speedDIAL display in
these cases.
5.1.7 Software Developer’s Kit (SDK)
The command list for X-Cite® 120LEDmini is available by request. To obtain the latest update,
please contact Excelitas Technologies.
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5.2 TTL
For high speed LED on/off control, TTL triggering can be used. The key thing to note about TTL
control in the X-Cite® 120LEDmini is: TTL mode MUST be enabled for the system to respond to a TTL
signal.
5.2.1 TTL Mode
TTL mode ensures that X-Cite® 120LEDmini’s cooling system and LED driver are active between
exposures, ensuring the fastest possible response to a TTL signal.
Enabling/disabling TTL mode can be accomplished manually via speedDIAL (refer to
section 4.3.4) or via computer commands (refer to SDK).
ON/OFF status of the system will be updated on speedDIAL when it is controlled via TTL.
5.2.2 TTL Mode Timeout
There is a potential disadvantage of TTL mode - in this “always active” state, the system
continues to consume energy and mechanical components accumulate wear. To help minimize
unnecessary consumption at the end of an imaging session (or if equipment is accidentally left
on), X-Cite® 120LEDmini is equipped with a TTL timeout setting. The factory default for the TTL
timeout setting is “Never”, but defining a timeout is recommended for any imaging session that
will end without an operator present to shut down equipment (e.g. middle of the night).
LED on
(response at maximum speed)
TTL low
time 4h,
TTL high
Timeout clock resets,
begins countdown
(unit stays active)
LED off
Time
elapses
4h
TTL mode times out
(drivers/cooling turn off, TTL
mode remains enabled)
TTL high
TTL low
LED on
(after drivers and cooling wake up)
Figure 8 Sequence of Events for TTL Mode Timeout (set to 4 hours)
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5.2.3 TTL Timing Diagram
This plot shows typical TTL timing values. These values should be used as a guide only. Actual
values will be dependent on the specific configuration/control hardware being used.
Interval
T1
Description
Delay time, TTL trigger on to LED on
Time (µs)
100 @ 100% power
T2
Delay time, TTL trigger off to LED off
30
5.2.4 TTL Signal and LED Status
TTL
High
Low
LED Status
ON
OFF
5.2.5 TTL Input Specifications
a.
b.
c.
d.
e.
Connector type: BNC (female port)
Maximum low level : +0.8V
Minimum high level : +2.2V
Maximum high level: + 5.5V
Typical input current: 800µA
5.3 Foot Pedal Control (Optional)
For hands-free operation, a foot pedal can be used to manually turn the LED on and off. The foot pedal
is an optional accessory and can be purchased separately from Excelitas Technologies.
1. Installing the foot pedal
a. Locate the “FOOT PEDAL IN” / “FP IN” input port on the rear panel of X-Cite®
120LEDmini.
b. Insert foot pedal plug.
c. Place foot pedal on floor.
2. Operating the foot pedal
a. Press and release pedal with your foot to toggle LED on and off.
b. On/off status of LED will be shown on speedDIAL display.
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c. If desired, the foot pedal can be used in combination with speedDIAL (e.g. turn on with
speedDIAL, turn off with foot pedal.) The ON/OFF status of the system will be reflected
on speedDIAL’s home screen whether it is changed via the foot pedal or by any other
means.
6 Troubleshooting
Organized by symptom, this section provides basic troubleshooting information for installation and setup parameters. X-Cite® 120LEDmini may be serviced by authorized technical personnel only.
6.1 Error Messages
If X-Cite® 120LEDmini detects a problem, an error message with one of the following codes will appear
on the speedDIAL display.
Error Code
1
3 or 5
6
Description
LED has exceeded maximum
operating temperature
Internal Error
LED is below minimum
operating temperature
Action
Turn off the system and wait for the LED to cool
down. Verify that the system has been installed with
adequate clearance for ventilation, especially
around vents on miniCUBE and LED Head. If fan is
not running or the problem persists contact
technical support for assistance.
Power cycle the X-Cite® 120LEDmini unit. If the
error message reappears contact technical support
for assistance.
Ensure the room temperature is within the
recommended operating limits. If unit has been
stored / transported in a cold environment, allow it
to warm up to room temperature and restart. If the
problem persists contact technical support for
assistance.
6.2 Failure to Power Up
If X-Cite® 120LEDmini fails to POWER up or function properly, use the following checklist to eliminate
the most common causes of problems. Check for the following:
1. Power Connection Check:
a. The power supply cord is securely connected to a grounded (earthed mains socket)
functional outlet.
b. The power supply cord is securely connected into the 3-pin outlet on the rear of the
controller unit.
c. The main AC power switch is in the ON position.
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2. Fuse Check:
a. Check both main power fuses. First disconnect the power cord, then carefully remove
the fuse tray assembly next to the AC receptacle on the rear of the unit. Note:
depending on the orientation of the AC receptacle, the fuse tray may be below or beside
it. The red arrow in figure 10 (A) shows where a flat screwdriver can be inserted to
gently lever out the fuse tray.
A
B
C
Figure 5 Location and Removal of Fuse Tray in AC Receptacle
b. If either or both of the fuses are open, replace with the same type (4A, 250V).
c. Note: To determine if a fuse is intact (i.e. OK) or open (i.e. blown), remove the fuse
from the tray and check with a multi-meter set to resistance (Ω). An intact fuse will read
“0Ω” (or another very low value), an open fuse will have an extremely high Ω reading.
d. Note: Fuses that chronically need replacing are usually a signal that something is wrong
and Technical Support should be contacted.
3. SpeedDIAL Check:
a. Verify that the pins on speedDIAL connector are straight.
b. Verify that speedDIAL is plugged securely into miniCUBE.
6.3 Low Illumination Intensity
1. SpeedDIAL Setting Check:
a. Verify that the LED intensity is set to a sufficient level.
b. Verify that the LED Head is turning on.
2. Microscope Check:
a. Verify that the Microscope Flange is appropriate for the microscope configuration.
Note: Some flanges have the same mechanical fitting but different optics.
b. Verify that everything in the microscope beam path is properly aligned and open, e.g.
shutters, apertures, diaphragms, filters, filter cubes, etc.
c. Verify that microscope filter sets are for the appropriate wavelengths.
d. Verify that air objectives are clean / immersion objectives have enough of the
appropriate fluid.
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6.4 Other Potential Symptoms & Questions
Category
speedDIAL
TTL Triggering
Audible Noise
Symptom
Favorite intensity setting: doubleclick turns off LED instead of going
to the stored setting
New settings are forgotten when
unit is shut down and powered up
Turning dial does not scroll through
the menu options
“Lock” icon is on display, speedDIAL
not responding to manual control
TTL signal does not trigger unit
TTL response time is longer than
usual
miniCUBE fan never turns off, even
when LED is not on
Action
Increase delay in the speed (Spd) setting.
After changing settings, wait at least five
(5) minutes before shutting unit down.
Turn dial in the opposite direction.
Send “unlock” command via computer OR
power down unit and restart.
Enable TTL mode.
Verify that timeout settings are
appropriately set.
Fan continues to run for five (5) minutes
after LED is turned off. If it runs longer,
disable TTL mode (it is likely enabled).
Note: The LED head has an additional feature of a temperature-controlled fan which only turns on when
required by the unit, thereby preventing additional vibration or acoustic noise which may interfere with
sensitive applications. The fan in the LED head will automatically turn on when the LED temperature
reaches 55°C and turn off when the LED temperature is below 45°C. Timing will vary depending on the
ambient temperature of the LED head’s location and duty cycle.
7 Routine Care and Maintenance
7.1 General
X-Cite® 120LEDmini is a very low maintenance system with no consumable components. By maintaining
the following conditions, performance will be maximized and risk of future problems will be reduced.
1. Maintain a clean work area, keeping the X-Cite® 120LEDmini air vents unobstructed.
2. Ensure that the cable connecting the miniCUBE and LED Head has some slack in it and is never
cut, stretched, kinked, or forced into a sharp bend.
3. If X-Cite® 120LEDmini must be moved, ensure that both the miniCUBE and the LED Head are
supported. Never use the cable as a handle.
4. Never leave the internal optics of the LED Head exposed. When not connected to a microscope,
ensure the output port is always covered by a Microscope Flange or the supplied protective
plastic cover.
5. Never touch optical surfaces with fingers, tools or any other abrasive/sticky/sharp materials or
fluids.
6. If cleaning is required, follow the directions in the next section.
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7.2 Cleaning - Exterior Surfaces
If necessary, exterior surfaces of X-Cite® 120LEDmini’s LED Head, miniCUBE and speedDIAL can be
cleaned with a mild soap and water solution and lint-free cloth.
1.
2.
3.
4.
Turn unit off and disconnect AC power prior to cleaning.
Use a damp cloth only - do not allow cleaning solution to get into I/O ports, air vents or seams.
Avoid optical surfaces.
Allow the unit to dry before turning it on.
7.3 Cleaning - Optical Surfaces
Cleaning of optical surfaces is not generally required. However, if any visible contamination or
fingerprints appear on the lens surface, cleaning may be necessary.
1. Recommended Cleaning Materials
a. Rubber bulb dust blower
b. Lint-free lens tissue, lint-free cotton swabs
c. Powder-free gloves or finger cots
d. Lens cleaning solution, reagent-grade isopropyl alcohol, or another appropriate solvent
2. Cleaning Procedure
a. Use rubber dust blower to blow off any loose lint, dust or other contaminant.
b. If the contaminant is a fluid (e.g. water, immersion oil), first use a dry lens tissue (or
cotton swab) to wick away as much as possible – do not wipe.
c. Saturate a corner of the lens tissue (or the cotton swab) with cleaning solvent, and
gently wipe the optical surface in one pass. Note: Take care not to “flood” the area
with solvent, particularly near unsealed joints (e.g. lens-retaining ring interface).
d. Repeat the previous step with a fresh portion of lens tissue (or new cotton swab) – this
will help avoid recontamination of the optical surface and minimize the amount of
cleaning required.
e. Let solvent evaporate and verify that the optical surface is clean. Repeat cleaning steps
as necessary.
f. Before reinstalling and/or using the optics, allow them to dry completely.
CAUTION: Before using any solvent, consult the manufacturer’s Material Safety Data Sheet (MSDS)
and your internal Health and Safety Advisor for proper handling, storage, and disposal instructions.
7.4 Thermal Management System
X-Cite® 120LEDmini’s unique thermal management system incorporates air cooling technologies. If the
general care and maintenance guidelines described above are followed, no further maintenance is
required to ensure continued superior performance.
If, through accident or negligence, the cable between miniCUBE and the LED Head is damaged:
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1. Power off and discontinue using the system.
2. Contact Technical Support (techsupport@excelitas.com).
8 Technical Specifications
8.1 General
Height
Width
Depth
Weight
miniCUBE
LED Head
110mm (4.4″)
135mm (5.3″)
180mm (7.1″)
100mm (3.9″)
a
230mm (9.1″)
110mm (4.4″)a,b
3.4kg (7.5lbs) miniCUBE and LED Head are
permanently connected
speedDIAL
59mm (2.3″)
80mm (3.1″)
112mm (4.4″)
0.3kg (0.7lbs)
Notes:
a. Does not include clearance required for cable, minimum 200mm (8″).
b. Does not include microscope flange, varies by type, approximately 10-15mm (0.4-0.6″).
8.2 Electrical
Power Supply
Input Voltage
Current
Input Surge
Protection
Fuse Rating
Power Factor Corrected, Universal Input
100-240VAC, 50/60Hz
2.0A max /100V, 1.0A max/240V
With cold start 40A/115V, 80A/240V
Over load and over temperature
Dual fuse system: each fuse rated at F 4.0A 250V
5x20mm type located in AC receptacle
8.3 Environmental – Operating Conditions
Ambient Temperature
Altitude
Atmospheric Pressure
Relative Humidity
Installation Category
Pollution Degree
Enclosure Rating
10° to 35° C
2000m max
700 to 1060 hPa
15 to 90% RH (non-condensing)
II
2
1
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8.4 Environmental – Transport and Storage Conditions
Temperature
Relative Humidity
Atmospheric Pressure
-35° to 60° C
10 to 95% RH (non-condensing)
500 to 1060 hPa
8.5 Input/Output (I/O) Connections
Connection
speedDIAL - IN/OUT
Connection Style
Mini DIN plug, 9pos
USB - IN/OUT
B
TTL - IN
Foot Pedal - IN
BNC
3mm stereo plug
Purpose
Communication between speedDIAL
and miniCUBE to control LED and
report status (on/off, intensity
adjustment, system error, etc.)
Communication between computer
and miniCUBE to control LED and
report status.
External trigger to turn LED on/off
External trigger to turn LED on/off
8.6 Output Stability
For maximum output stability, X-Cite® 120LEDmini should be set to a power level of 5% or greater. At
lower power levels (
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