X-Cite® XLED1 User Guide
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X-Cite® XLED1 User Guide
035-00465R rev. 7
X-Cite® XLED1 User Guide
035-00465R Rev.7
Made in Canada
Excelitas Canada Inc. 2015
All rights reserved
No part of this publication may be reproduced, transmitted, transcribed, stored in a retrieval
system or translated into any language in any form by any means without the prior written
consent of Excelitas Canada Inc.. Every effort has been made to ensure information in this
manual is accurate; however, information in this manual is subject to change without notice and
does not represent a commitment on the part of the authors.
Excelitas Canada Inc.
2260 Argentia Road
Mississauga ON L5N 6H7 CANADA
Trademarks
X-Cite® is a registered trademarks of Excelitas Canada Inc.. All rights reserved.
All other product names are trademarks of their respective owners. Any product or software
photos shown are for reference only and are subject to change without notice.
No part of this publication may be reproduced, transmitted, transcribed, stored in a retrieval system or
translated into any language in any form by any means without the prior written consent from Excelitas
Canada Inc.. Every effort has been made to ensure information in this manual is accurate; however,
information in this manual is subject to change without notice and does not represent a commitment on
the part of the authors.
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Table of Contents
1.
2.
Introduction ....................................................................................................................... 1
Safety ................................................................................................................................. 2
2.1
2.2
2.3
3.
Getting Started .................................................................................................................. 7
3.1
3.2
3.3
4.
Main LED Screen........................................................................................................................ 25
Set LED Triggers Screen ............................................................................................................ 27
Trigger Graphs Screen ............................................................................................................... 31
Maintenance Screen ................................................................................................................... 32
Dichroic Values ........................................................................................................................... 35
Alarm Information ....................................................................................................................... 35
Clearing alarms........................................................................................................................... 37
System Information ..................................................................................................................... 37
Operating the Optional Touch Screen Controller ......................................................... 38
7.1
7.2
7.3
7.4
7.5
7.6
7.7
7.8
7.9
8.
General Installation ..................................................................................................................... 11
LED Module/Driver and Dichroic Installation .............................................................................. 12
Installing Excitation Filter Holders .............................................................................................. 16
Graphical User Interface (GUI). .................................................................................................. 18
Light Guide Auto-Detect ............................................................................................................. 19
Inserting/Removing Light Guide ................................................................................................. 19
Installing the Collimating Adapter ............................................................................................... 21
Powering Up/Powering Down ......................................................................................... 22
Operating the GUI Control Panel.................................................................................... 24
6.1
6.2
6.3
6.4
6.5
6.6
6.7
6.8
7.
XLED1 Front Panel ........................................................................................................................8
XLED1 Back Panel ........................................................................................................................9
Optional Touch Screen Controller/ Swivel Stand ....................................................................... 10
Installation Procedures................................................................................................... 11
4.1
4.2
4.3
4.4
4.5
4.6
4.7
5.
6.
Glossary of Symbols ......................................................................................................................2
Safety Precautions.........................................................................................................................2
Principles of ESD Control ..............................................................................................................5
Log-In Screen ............................................................................................................................. 39
Main LED Screen........................................................................................................................ 41
Edit LED Name Screen .............................................................................................................. 43
Set LED Triggers Screen ............................................................................................................ 44
Trigger Graphs Screen ............................................................................................................... 48
Maintenance Screen ................................................................................................................... 49
Alarm Information ....................................................................................................................... 51
Clearing alarms........................................................................................................................... 53
System Information ..................................................................................................................... 54
Live Cell Mode (on Live Cell Configuration units) ........................................................ 56
8.1
Enabling Live Cell Mode ............................................................................................................. 56
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8.2
9.
Changing duty cycle in Live Cell Mode....................................................................................... 57
External Control .............................................................................................................. 58
9.1
9.2
9.3
10.
11.
12.
External Trigger Interface ........................................................................................................... 58
Trigger Input/Sync Output Signals Requirements ...................................................................... 60
I/O Signal Descriptions ............................................................................................................... 60
LED Module and Driver Replacement ........................................................................ 62
Dichroic Replacement ................................................................................................. 65
Routine Care and Maintenance................................................................................... 67
12.1
12.2
12.3
12.4
12.5
13.
14.
15.
16.
17.
18.
19.
20.
General ................................................................................................................................... 67
Cleaning Procedure, XLED1 & Touch Screen Controller: ...................................................... 67
Handling and Cleaning of the LED Lens Assembly ................................................................ 68
Handling and Cleaning of the Dichroic ................................................................................... 69
Tips to Prevent Premature Degradation of Light Guides ........................................................ 71
Troubleshooting Symptom/Possible Cause .............................................................. 73
Technical Specifications ............................................................................................. 75
Environmental Conditions .......................................................................................... 77
Regulatory Compliance ............................................................................................... 77
Warranty ....................................................................................................................... 80
Warranty Table ............................................................................................................. 81
Component Number Table .......................................................................................... 82
Contact Information..................................................................................................... 83
Figures:
Figure 1 XLED1 Front Panel .................................................................................................... 8
Figure 2 XLED1 Back Panel Image.......................................................................................... 9
Figure 3 Touch Screen Controller ......................................................................................... 10
Figure 4 LED Module/Driver and Dichroic Locations .......................................................... 12
Figure 5 Locking Hub............................................................................................................. 13
Figure 6 Dichroic Replacement ............................................................................................. 15
Figure 7 Typical XLED1 LED Module .................................................................................... 16
Figure 8 Inserting filter into filter holder ............................................................................... 16
Figure 9 Attaching filter assembly to module ...................................................................... 17
Figure 10 Light Guide Port .................................................................................................... 20
Figure 11 Collimating Adapter............................................................................................... 21
Figure 12 Main LED Screen (GUI) .......................................................................................... 25
Figure 13 LED Trigger Screen (GUI)...................................................................................... 27
Figure 14 Trigger Graphs Screen (GUI) ................................................................................ 31
Figure 15 Maintenance Screen (GUI) .................................................................................... 32
Figure 18 Dichroic Database (GUI) ........................................................................................ 34
Figure 19 Alarm Code Screen (GUI) ...................................................................................... 36
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Figure 20 System Information (GUI)...................................................................................... 37
Figure 21 Log-In Screen (Touch Screen Controller) ............................................................ 39
Figure 22 Main LED Screen (Touch Screen Controller) ....................................................... 41
Figure 23 LED Name Edit Screen (Touch Screen Controller).............................................. 43
Figure 24 LED Trigger Screen (Touch Screen Controller) ................................................... 44
Figure 25 Trigger Graphs Screen (Touch Screen Controller) ............................................. 48
Figure 26 Maintenance Screen (Touch Screen Controller) ................................................. 49
Figure 28 Alarm Code Screen (Touch Screen Controller) ................................................... 51
Figure 29 System Information (Touch Screen Controller) ................................................... 54
Figure 30 Terminal Block Connectors .................................................................................. 58
Figure 31 Locking Hub ........................................................................................................... 62
Figure 32 LED Module and Driver Replacement .................................................................. 64
Figure 33 Dichroic Replacement ........................................................................................... 66
Tables:
Table 1 Front Panel LED Indicators ........................................................................................ 8
Table 2 Alarm Codes (GUI) .................................................................................................... 35
Table 3 Error Pop-up Screen Descriptions (GUI) ................................................................. 36
Table 4 Error Window Descriptions (GUI) ............................................................................ 38
Table 5 Alarm Codes (Touch Screen Controller) ................................................................. 51
Table 6 Error Pop-up Screen Descriptions (Touch Screen Controller) .............................. 52
Table 7 Error Window Descriptions (Touch Screen Controller) .......................................... 55
Table 8 External Trigger Pin Assignments ........................................................................... 59
Table 9 External Sync Out Pin Assignments (OUTPUT 5.5V, 8mA max) ............................ 59
Table 10 China RoHS ............................................................................................................. 79
Table 11 System Components Warranty .............................................................................. 81
Table 12 Component Part Numbers ..................................................................................... 82
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1.
Introduction
The X-Cite® XLED1 System is intended for use in fluorescence microscopy illumination. It
allows researchers the ability to excite fluorescence in samples being studied and
characterize their location or behaviour.
The X-Cite® XLED1 System resets the standard in LED fluorescence microscopy
illumination systems. The heart of the X-Cite® XLED1 System is the LED
Modules/Dichroics available for use in multiple configurations and combinations, each with
20,000 hour guaranteed lifetime. The systems’ ease of use is made possible by the
innovative solid state LED technology incorporated into the design of the system along
with simple to advanced triggering controls, plug and play Modules and system
intelligence. An easy-to-use Graphical User Interface (GUI) module allows full control of
each LED. Also available is the optional Touch Screen Controller, providing the full control
and monitoring of each LED module as never before seen in such a form factor.
Refer to the website for all LED module/driver and Dichroic locations and
combinations.
http://www.excelitas.com/Pages/Product/X-Cite-LED-Module-Configurator.aspx
The X-Cite® XLED1 System joins the Excelitas Technologies family of light systems
offering the same high level of innovation, quality and reliability that our customers have
come to expect.
Excelitas Technologies Corp. is a global technology leader focused on delivering
innovative, customized solutions to meet the lighting, detection and other highperformance technology needs of customers. X-Cite®, formerly of Lumen Dynamics (which
was acquired by Excelitas Technologies Corp. in November 2013) offers the Life Science
and Analytical Instrumentation market a broad range of innovative lamp and LED
fluorescence illumination and measurement solutions.
Excelitas recommends reading this manual to discover all the features available for the XCite® XLED1 System.
Please ensure that you register your XLED1 at:
http://www.excelitas.com/Pages/Support/Register_OX.aspx
X-Cite® XLED1
Enclosure containing all opto-mechanical assemblies, control electronics and port
interfacing.
Touch Screen Controller (optional)
Separate (remote) enclosure with touch screen display module for control of the XLED1.
Thank you for choosing X-Cite®!
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2.
Safety
2.1 Glossary of Symbols
Caution risk of danger – consult accompanying documents
Input/Output Signals
Input Signal
Output Signal
RISK GROUP 2
CAUTION
Possibly hazardous optical radiation/UV emitted from this product.
Use appropriate shielding
IEC 62471-2:2009
For detailed information contact Excelitas Canada Inc.
This product has been tested to the requirements of CAN/CSA-C22.2 No. 61010-1,
second edition, including amendment 1, or a later version of the same standard
incorporating the same level of testing requirements.
ATTENTION
Electro Static Discharge (ESD)
Observe precautions for handling Electrostatic Sensitive Devices
2.2 Safety Precautions
This series of cautions and warnings relate to the operation and maintenance of the XCite® XLED1 System. They are also presented throughout this User’s Guide where
necessary.
RISK GROUP 2
CAUTION
Possibly hazardous optical radiation/UV emitted from this product. Use appropriate
shielding. IEC 62471-2:2009
WARNING
Visible and invisible radiation is emitted from the aperture of the Light Guide- avoid
exposure to beam. Never look into the light emitting end of the Light Guide. The light could
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severely damage the cornea and retina of the eye, if the light is observed directly. Eye
shielding and protective clothing must be used at all times to protect exposed skin.
CAUTION
Use of the XLED1 system is intended only for microscopes using Excelitas Technologies
collimating adaptors. Any attempt to use this system with a microscope not approved by
Excelitas Technologies is not recommended and may impair system safety.
WARNING
Always make sure the Light Guide is properly inserted into the XLED1 and into the
microscope, prior to turning on power to the unit. This will minimize the risk of exposure to
UV light.
CAUTION
Removal of the service cover is intended for qualified technical personnel only!
No user serviceable parts are located within the XLED1 chassis or the Touch Screen
Controller. This system contains hazardous voltages-do not attempt to remove access
covers while equipment is energized.
WARNING
To reduce the risk of fire or shock, always replace the AC mains fuses with the same type
and rating (F6.3A, 250V).
Ensure that the X-Cite® XLED1 System external power supply cord is connected to a
grounded (earthed mains socket) 3-pin outlet only! Use only the power supply cord set
provided with the system. Substitution is not recommended; operation and/or performance
may be affected and may impair operating safety.
WARNING
The Level of UV and visible energy supplied by the X-Cite® XLED1 System is sufficient to
ignite flammable substances. During installation in an automated environment the
appropriate measures must be included to prevent any such occurrence.
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WARNING
Should the X-Cite® XLED1 System be used in a manner not specified by Excelitas
Technologies, the protection provided by the equipment may be impaired. Do not
attempt to connect the Touch Screen Controller to any type of electrical equipment other
than the XLED1 main system as indicated in Figure 2 of this manual.
WARNING
This unit is designed for bench top use only! Always ensure that the unit is operated on a
hard, stable surface. This will prevent obstruction of the bottom chassis ventilation
openings. Any obstruction of these openings could result in a possible over-heating
condition. Do not attempt to remove or tamper with the rubber feet located on the bottom
of the unit.
CAUTION
Use of controls or adjustments or performing procedures other than those specified
herein may result in hazardous radiation exposure.
The Light Guide operation can be affected if handled incorrectly. Never touch the light
emitting end of the Light Guide. The presence of skin oils may result in a decrease in
system performance.
Always ensure the system power is turned off, prior to disconnecting or re-connecting
the Light Guide.
CAUTION
To prevent damage/ degradation of the Light Guide, always allow adequate clearance at
the front of the XLED1 unit to prevent kinking or excessive bending.
WARNING
Do not lift the XLED1 from the rear of the removable housing when system is either on
or off.
CAUTION
To protect the LED module, driver and system from electrostatic related damage, which
can disable the system, permanently damage the LED module & driver, or require repair
by service technicians, place all Modules on an antistatic surface, such as an antistatic
discharge mat, an antistatic bag, a disposable antistatic mat or a grounded metal
surface. If possible, wear an antistatic grounding strap connected to a metal surface on
the chassis when you replace any LED module or LED driver. Refer to section 2.3 for
understanding the principles of ESD control.
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2.3 Principles of ESD Control
The basics of electrostatic charge, discharge, types of failures, ESD events, and device
sensitivity may be summarized as follows:
Virtually all materials, even conductors, can be triboelectrically charged, (an
electric charge generated by friction).
The level of charge is affected by material type, speed of contact and separation,
humidity, and several other factors.
Electrostatic discharge can create catastrophic or latent failures in electronic
components.
Electrostatic discharge can occur throughout the manufacturing, test,
shipping/handling, or operational/repair processes.
Component damage can occur as the result of a discharge from the component
as well as a direct discharge to the component.
Components vary significantly in their sensitivity to ESD.
The best method of preventing ESD is to use an ESD wrist strap, a grounding mat or a metal
table. However, since most users do not have access to such items, we have provided the
following guidelines to help reduce the risks and levels of ESD:
Zero Potential - Most importantly, make sure you and the system are at Zero
Potential by continuously touching an un-painted metal surface of the chassis or
the grounded table.
Standing - It is also very important that you are standing at all times when
working on the system. Sitting on a chair can generate more electrostatic energy.
Cords - Make sure everything is removed from the back of the system (power
cord, touch controller, interface cables, etc).
Clothes - Make sure not to wear any clothing that conducts Electrical Charge,
such as a wool material.
Weather - Electrical storms can increase the ESD risk; unless absolutely
necessary, try not to work on the system during an electrical storm.
Accessories - To help reduce ESD and help prevent other problems, it is also a
good idea to remove all jewellery.
With this basic understanding of ESD and its impact on your environment, you can then begin to
develop an effective ESD control program by reducing as many static generating processes or
materials as possible, such as the contact and separation of dissimilar materials and common
plastics from the work environment. Keep other processes and materials at the same
electrostatic potential. Electrostatic discharge does not occur between materials kept at the
same potential or at zero potential. Therefore, provide ground paths, such as a wrist strap or a
conductive surface connected to ground, to allow static accumulating in yourself and/or
materials nearby to discharge to ground rather than to critical components.
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Prevent discharges that do occur from reaching susceptible parts and assemblies. Package and
transport susceptible devices in proper packaging and materials-handling products. These
materials may effectively shield the product from charge, as well as reduce the generation of
charge caused by any movement of product within the container. Such products include ESD
shielded bags, conductive totes, and antistatic packaging materials.
Helpful ESD Links:
http://en.wikipedia.org/wiki/Electrostatic_discharge
http://en.wikipedia.org/wiki/Electrostatic_sensitive_device
http://www.ipc.org/ContentPage.aspx?pageid=IPC-and-ESDA-Collaborate-onRequired-ESD-Training-for-Electronics-Assembly-Operators
http://www.ce-mag.com/99ARG/ESD%20Assoc175.html
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3.
Getting Started
System Components
Carefully unpack the system and accessories. As you remove each item from the box
place a check mark next to each item to ensure that all ordered components are present.
Store the packaging and packing materials for future use.
Box Contents Check List
1. XLED1 + ordered modules and dichroics …..………………….…
2. AC Cord ………………………………….…………………………..
3. USB Cable..……………….………………......................................
4. XLED1 Locking Hub Key………………………………………….....
5. Locking Hub Key Hanger (refer to Figure 2 for install location)....
6. XLED1 Quick Start Guide
(User Guide, Software and Driver downloads:
http://www.excelitas.com/Pages/Product/X-Cite-XLED1.aspx)….....
7. Product Registration Card .….………………………………………
8. XLED1 Excitation filter holders (set of 4)…………………………..
9. XLED1 trigger cable………………………………………………….
Please go online to register at http://www.excelitas.com/Pages/Support/Register_OX.aspx
Refer to Table 12 for individual part numbers
If your packaged system is missing any of the listed components, call Excelitas
Technologies at (905) 821-2600 or Toll Free 1-800-668-8752.
Refer to the website for all LED module/driver and Dichroic locations and
combinations.
http://www.excelitas.com/Pages/Product/X-Cite-LED-Module-Configurator.aspx
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3.1 XLED1 Front Panel
LIGHT GUIDE INDICATOR
IIINDICAINDICATOR
LIGHT GUIDE PORT
HOUSING LOCKING HUBS
(BOTH SIDES)
FUTURE EXPANSION
PORT (NOT FOR USE)
MASTER POWER ON/OFF
LED ON/OFF BUTTON/LED
SWITCH
ON INDICATOR
Figure 1 XLED1 Front Panel
LED Indicator
Status
Description
Light Guide LED
Green
Red
Yellow
Light Guide fully inserted
Light Guide not fully inserted
Live Cell Mode
LED ON
Green
No Illumination
Enabled (On)
Disabled (Off)
Table 1 Front Panel LED Indicators
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3.2 XLED1 Back Panel
LOCKING HUB KEY HANGER,
EXHAUST FAN
RECOMMENDED LOCATION
TRIGGER INPUT PORT
SYNC OUT PORT
USB PORT
EXHAUST FAN
AC CONNECTION/FUSE
TRAY
DISPLAY MODULE PORT (TOUCH
SCREEN CONTROLLER)
LOCKING HUB KEY HOUSING
OPTIONAL LOCATION
Figure 2 XLED1 Back Panel Image
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3.3 Optional Touch Screen Controller/ Swivel Stand
TOUCH SCREEN
X-CITE® BUTTON
SWIVEL LEG
Figure 3 Touch Screen Controller
The optional Touch Screen Controller has a stand made up of two separate extendable legs
that swivel out from the back of the Touch Screen Controller. Refer to Figure 3.
To swivel the legs out, push in the green X-Cite® button and swivel the leg out to one of the preset stops to achieve the optimal viewing angle. Repeat for the other leg.
To retract the legs push in the green X-Cite® button and swivel the legs back into the body of
the controller.
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4.
Installation Procedures
4.1 General Installation
Note: Before any installation occurs ensure there is a minimum space allowance of
50mm surrounding XLED1 to maintain acceptable airflow performance. Airflow
is exhausted from the rear of XLED1.
Excelitas Technologies recommends installing the locking hub key hanger. Bend the flexible
tang into a hook shape, peel off the protective backing and affix to the recommended location
shown on the rear panel in Figure 2.
Do not use the removable cover as a means to lift the XLED1. It is recommended that the
XLED1 and the optional Touch Screen Controller are mounted on flat stable surface/platform
adjacent to microscope.
If purchased, connect the optional Touch Screen Controller to the display module connection
provided at the rear of the XLED1 panel. Refer to Figure 2.
Note: Do not use both the GUI Control Panel and the Touch Screen Controller
at the same time.
If Triggering is required, connect the 15pin HD-DB15 trigger I/O breakout cables to the trigger
input and sync output connections located on the back XLED1 panel, refer to Figure 2.
If interfacing with the GUI Control Panel, connect the computer to XLED1 via the USB
connection located on the back panel.
Connect the AC cable to the AC connector on the back of the XLED1 panel.
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4.2 LED Module/Driver and Dichroic Installation
The XLED1 system ships with the LED Modules, Drivers and Dichroics uninstalled and in
separate boxes. These need to be installed according to the instructions below.
On line configurator at http://www.excelitas.com/Pages/Product/X-Cite-LED-ModuleConfigurator.aspx for module configurations and locations.
“Location” refers to the positions identified in Figure 4, showing an empty X-Cite® XLED1 unit.
(yellow = Dichroic location, blue = LED module and driver location). For LED Module/Driver and
Dichroic Replacement instructions, refer to sections 10 and 11.
Dichroic Slot DC3,4
Driver Slot 3
LED Module Slot 4
Driver Slot 4
LED Module Slot 3
Driver Slot 1
Driver Slot 2
LED Module Slot 2
Dichroic Slot
DC1,2,3,4
Dichroic Slot DC1,2
LED Module Slot 1
Figure 4 LED Module/Driver and Dichroic Locations
CAUTION
To protect the LED Module, Driver and system from electrostatic discharge (ESD)
related damage, which can disable the system, cause latent defects or permanent
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damage, or require repair by service technicians, please take the following ESD
prevention steps:
Ensure the unit is unplugged
Ensure you are properly grounded, ideally by using an ESD grounding strap attached
to an ESD protective surface.
Refer to section 2.3 for principles of ESD and additional ESD precautions.
Tools Required:
Locking Hub Key (supplied)
#2 Philips Head Screwdriver (not supplied)
Torque tool (not supplied)
LED Modules, Drivers and Dichroics are shipped in ESD bags.
LED Modules are shipped with a protective lens cap covering the LED lens.
Ensure the system is OFF and the AC power cord is unplugged from the power source.
Using the locking hub key supplied, rotate the green locking hubs 90 degrees and remove the
locking hubs. Holding the XLED1 housing at the back, slide the XLED1 housing up and back
(away from the fascia) for removal. The locking hub key can be stored on the locking hub key
hanger, located on the back panel of XLED1.
Figure 5 Locking Hub
LED Modules are shipped with their unique Drivers. Both components must be installed at the
same time. Refer to Figure 4 for module and Dichroic locations.
Caution: Do not touch the LED lens surface. Skin oils and scratches can cause the
optical output to decrease.
Caution:
The Dichroic window operational life can be significantly shortened if it is handled
incorrectly. Do not touch the window surface. Skin oils and scratches can cause the
window to fail prematurely. Refer to section 12.3 for handling and cleaning
instructions.
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To install the LED Module and Driver, first remove the protective LED lens cap from the LED
Module. Align the LED module/driver male pins with the female socket connection and gently
push down into place. Tighten the Philips-Head screws until a positive force is felt and apply ¼
turn. DO NOT OVER TIGHTEN THE SCREWS. (If using a calibrated torque-meter, Excelitas
Technologies recommends a torque value of 5.7 lb-in/± 0.3 lb-in.)
If there is a mismatch installation of either standard powered or high powered Drivers, the
corresponding LED will not operate.
Install the Dichroics by inserting through the slot and lightly press down until a positive force is
felt. Ensure to hand tighten the locking screw, do not use mechanical tooling such as
screwdrivers, pliers etc. Refer to Figure 4 for Module and Dichroic locations.
If the Dichroic is inserted incorrectly, the Dichroic’s orientation key (located on the frame), will
not pass through the cut-out on the top plate, resulting in an unseated Dichroic. Refer to Figure
6.
When changing a Dichroic the Edge value must be manually entered into the system. If using
the GUI Control Panel Refer to section 6.4 and Figure 18. For instructions on changing the
Edge, reflect and transmit values.
If using the Touch Screen Controller, to change the Edge value, touch on the Dichroic to see the
pop-up window, touch the top tab of the window and an edit screen will appear. Type in the new
Edge value and press “GO” to return to the maintenance screen. The edge value is printed on
the Dichroic module. For new Dichroics, the reflect and transmit values will not be automatically
updated, refer to
section 6.5 for these values.
Replace the XLED1 housing ensuring a snug fit. Insert the locking hubs and using locking hub
key supplied, rotate the green locking hubs to the locked position. Return the locking hub key to
the locking hub key hanger, located at the rear of the system. The system will not allow the main
power switch to function if the housing is not replaced properly.
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LOCKING SCREW
ORIENTATION KEY
DICHROIC WINDOW
ORIENTATION CUT-OUT
DICHROIC
MODULE
Figure 6 Dichroic Replacement
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4.3 Installing Excitation Filter Holders
All XLED1 units include 4 filter holders that accommodate any standard 25mm band-pass filter.
Additional filter holders can also be ordered separately by using part number 019-00175R.
Figure 7 Typical XLED1 LED Module
Follow instructions as per section 4.2 of the XLED1 user manual to open the XLED1 system and
remove the LED module of interest. Only remove the LED module, there is no need to remove
the driver.
NOTE: care must be taken to not contaminate or damage the lens or excitation filter(wearing
gloves is recommended when performing this procedure).
Place the filter holder on a flat clean surface as shown below.
With the arrow (indicating the coated side of the filter) pointing upward, place the filter inside the
filter holder (Figure 8) and tighten the set screw with supplied allen key
Figure 8 Inserting filter into filter holder
Attach the excitation filter + filter holder into the LED module lens as depicted below and secure
the set screw with the provided allen key
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Figure 9 Attaching filter assembly to module
Re-insert the LED module into the XLED1 system.
Note: Utmost care must be taken to not contaminate the lens or the
Excitation filter. e.g., finger prints, dust, etc. The use of gloves or other
material suitable for handling optics is recommended.
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4.4 Graphical User Interface (GUI).
Note: Ensure user is logged in as Administrator. The USB drivers and GUI can be
downloaded from our website under the “SOFTWARE DOWNLOADS” section of the
Resource Centre. http://www.excelitas.com/Pages/Product/X-Cite-XLED1.aspx
System Requirements:
Operating System:
Windows XP 32/64bit, Windows Vista 32/64bit, Windows 7 32/64bit,
Windows 2003 Server 32/64bit, Windows 2008 Server 32/64bit, Windows
2008 R2.
Minimum Hard Drive Space: 5MB
1. Installation of USB Drivers (from web/windows update)
1.
2.
3.
4.
5.
6.
7.
8.
Log on to internet.
Connect USB cable from XLED1 to computer.
Turn on XLED1.
“Found New Hardware” will appear on the computer screen.
“Found New Hardware Wizard” will appear on the computer screen.
Select “Install Software Automatically (Recommended)” click “Next”.
Once wizard finds software click on “Finish”.
Follow instructions for GUI Installation.
Note: If window pops up with “search on-line for driver” click yes.
2. Alternative Installation Option
Use MS Windows update as per your computer operating system.
Look for:
1. “Excelitas Technologies Hardware XLED1”.
2. Follow download instructions.
3. Repeat for 2nd Driver (1st install is USB Base Driver, 2nd install is Virtual
Serial/com Port.)
4. Locate downloaded Drivers in your Device Manager.
5. Follow instructions for GUI Installation.
Note: Ensure Installation of USB Drivers has been completed before installing the
GUI.
3. Installation of GUI Control Panel (from ZIP File):
1. Connect to the internet.
2. Download GUI Control Panel Software from our website:
http://www.excelitas.com/Pages/Product/X-Cite-XLED1.aspx
3. The USB Driver must be installed first. Refer to paragraphs 1or 2 in this
section.
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4. In your downloads folder, search for and open the “032-00136.zip” file.
5. Double click on SETUP.EXE
6. Next follow the setup instructions as they appear by clicking “next” each time
the user prompt appears, until the installation has been completed and
“finish” appears. Click on “finish” to complete the installation.
Note: A Software Development Kit (SDK) 035-00469R is available upon request.
This SDK will permit computer control of functions described in the serial
command sets. Please contact Excelitas Technologies to request this
document,
(refer to section 20).
4.5 Light Guide Auto-Detect
The system will not allow the enabling of LED sources if there is not a Light Guide present in the
receptacle.
The system will disable all LED sources if the Light Guide has been detected as “removed” from
the unit. Refer to section 4.6 for Light Guide installation.
The lightguide LED on the front panel of XLED1 will illuminate green indicating the Light Guide
insertion is correct. If the Light Guide is not fully inserted the Light Guide indicator LED will
illuminate RED, (there will be 3 beeps from the optional Touch Screen Controller). The error
icon will illuminate on the GUI Control Panel or on the Touch Screen Controller.
The system will not allow the main power switch to function if the housing is not replaced
properly. When replacing the XLED1 service housing, ensuring a snug fit and using locking hub
key supplied, rotate the green locking hubs to the locked position.
4.6 Inserting/Removing Light Guide
Ensure that the main power is off on the XLED1 unit.
Ensure the power cord is not connected to XLED1.
Ensure that the protective end caps are removed from both the input and output ends of the
Light Guide prior to installation and remove plastic plug (if present) from the Light Guide port on
the front panel of XLED1.
Hold the Light Guide by the grey sleeve/strain relief portion only, insert the Light Guide into the
Light Guide port. Push the Light Guide in until it seats with a positive "click" (up to the “A” line
printed on the grey ferrule).
Note: Never grip the Light Guide during installation or removal in a place other
than the reinforced strain relief portion of the Light Guide.
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LIGHT GUIDE LED INDICATOR
LIGHT GUIDE PORT
Figure 10 Light Guide Port
During use, when the Light Guide is fully inserted the Light Guide LED indicator will illuminate
green indicating full Light Guide insertion. If the Light Guide is not fully inserted when the unit is
powered up, the Light Guide indicator LED will be RED, (there will be 3 beeps from the optional
Touch Screen Controller). The error icon will illuminate on the GUI Control Panel or on the
Touch Screen Controller.
To remove the Light Guide, firmly grip the strain relief near the Light Guide retainer and pull out
firmly.
Note: XLED1 is designed for use with a 3mm liquid Light Guide. Excelitas
Technologies cannot guarantee the performance of the system if using
Light Guides other than those supplied by Excelitas Technologies.
Note: Refer to section 12.5 for tips to prevent premature degradation of Light
Guides.
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4.7 Installing the Collimating Adapter
Ensure that the main power is off on the XLED1 unit.
The existing lamp housing must be removed from the microscope before the Collimating
Adapter can be mounted. Do not discard the existing mounting hardware, since it will be used to
secure the Collimating Adapter to the microscope.
Remove the Collimating Adapter from its packaging.
Note: The Collimating Adapter has been set at the factory. No adjustments by
the customer are required; however some adapter models are outfitted
with centering set screws, for longer light path applications. Separate
instructions are included with these models.
Insert the flange portion of the Collimating Adapter into the lamp port of the microscope. Using
the existing hardware, tighten the screw(s) until the flange is fully secured.
Note: The installation of the Collimating Adapter will vary with microscope
models.
Remove the protective cap from the output end of the Light Guide. Insert the Light Guide into
the input portion of the Collimating Adapter until it is flush with the adjustable insert. Secure the
Light Guide to the Collimating Adapter by tightening the thumbscrew. Do not over tighten.
Note: The Light Guide has a minimum bend radius of 1.6 inches (40.0mm).
Bending or coiling the Light Guide tighter than this radius will result in
permanent damage to the Light Guide.
Figure 11 Collimating Adapter
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5.
Powering Up/Powering Down
Note: Use UV Safety Eye Glasses before powering up the system.
The system will be fully operational under fluorescent or incandescent lighting.
The system will be fully operational under zero lighting (darkness).
The system will be fully operational in any daylight level other than direct incident
sunlight.
Ensure that the Light Guide has been properly installed as per instructions in section 4.6. If the
Light Guide is not fully inserted the Light Guide indicator LED will be RED, (there will be 3 beeps
from the optional Touch Screen Controller). The error icon will illuminate on the GUI Control
Panel or on the Touch Screen Controller.
Ensure the master power switch is OFF. Plug the XLED1 power adaptor into a properly
grounded electrical outlet.
Push the master power switch to ‘ON’ and ensure that the internal fans are operational. The
cooling fans will remain functional throughout the complete start-up to power-down cycle.
The LED ‘ON’ indicator light will be off (No Illumination).
Open the GUI Control Panel at this time or if the optional Touch Screen Controller is connected,
wait for the XLED1 logon screen.
Note: Do not use both the GUI Control Panel and the Touch Screen Controller
at the same time.
Note: Do not connect the XLED1 Touch Screen Controller when the XLED1 is
powered on.
The system will commence with a Power-On-Self-Test (POST). All outputs will be inactive
and all inputs will be unresponsive during the POST cycle, which takes approximately 60
seconds. In the event of a POST error, the system will report the error and either allow the user
to proceed or halt, depending on the error condition. Refer to section 7.7 for alarm information if
using the Touch Screen Controller. Refer to section 6.6 for alarm information if using the GUI
Control Panel.
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During the POST sequence the system stabilization will:
Check for number of LED Modules installed.
Read LED module ID, check hours and check System LED NVM write capability.
Ensure LED and driver type match.
Check LED module thermistor.
Check for alarms.
Check for Pulse Generator Module.
Check for connected Light Guide.
Check for connected Touch Screen Controller.
If using the GUI Control Panel, once the system runs through and has exited the POST
sequence the system is ready to use, there is no reset or warm up sequence required.
To power down the system after use, ensure all LEDs are off, click on the log out pad on the
GUI Control Panel and push in the master power button to turn the XLED1 system off.
All previous settings in the GUI Control Panel will be saved.
If Using the Touch Screen Controller:
If using the optional Touch Screen Controller, once the system runs through and has exited the
POST sequence the Touch Screen Controller screen will turn on automatically and the system
is ready to use, there is no reset or warm up sequence required. If the Light Guide is not fully
inserted the Light Guide indicator LED will be RED and there will be 3 beeps from the Touch
Screen Controller and the light guide icon will illuminate at the top of the screen.
To enter the system at the Log-In Screen, follow the instructions in section 7.1.
If logged in as a ‘Guest’ data will not be saved after completion of session.
Before powering down the system navigate to the Main LED Screen (section 7.2) and press the
LOG-OUT pad. This will ensure all data entered from the latest session is saved. Failure to
logout before powering down the system will result in loss of all recently changed settings.
To power down the system after use, ensure LED button is OFF, push the master power switch
to the ‘OFF’ position.
Note: Ensure that users log-out of the Touch Screen Controller before
powering down or all entered data from the latest session will not be
saved. (refer to section 7.2). The system is defaulted NOT to save any
data when logged in as ‘Guest’ regardless of logging out or not.
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6.
Operating the GUI Control Panel
Screen Sitemap:
Main LED Screen
(refer to section 6.1)
Set LED Triggers
(refer to section 6.2)
Trigger Graphs
(refer to section 6.3)
Maintenance
(refer to section 6.4)
Alarm Information
(refer to section 6.6)
System Information
(refer to section 6.8)
Note: all screens have a top bar indicator
Touch Screen Locked:
Disabled.
Light Guide Inserted:
Illuminates when the Light Guide is properly inserted.
Alarm:
Indicates a system alarm, user should navigate to the
Alarm Screen to determine the source of the alarm.
LED on:
Illuminated when any LED Module is on.
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6.1 Main LED Screen
Figure 12 Main LED Screen (GUI)
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The main screen displays a section for each LED module (e.g: UVX, BDX, BGX and RLX),
these can be renamed to desired fluorophores. Click on the desired wavelength name to view
the wavelength range or to rename the LED. Enter a name or number and press OK. The
display will now be changed.
Each LED module section has Unlocked (grey), Locked (green), OFF (grey), and ON (green)
features. The Unlock/Lock pads, unlock or lock that individual LED module section. In lock
mode all settings (coarse and fine intensity, percentage window and wavelength window) for
that LED module are locked and cannot be changed.
Each LED module section has a coarse intensity adjustment slider and fine intensity adjustment
“UP” and “DOWN” pads. The fine intensity adjustment arrows allow intensity adjustments
from 5% to 100% in 0.1% increments. A numerical value can be entered by clicking on the %
value to access the popup Edit Entry window. Enter a number and press OK. The intensity will
now be changed.
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The main screen has a global “LOCK ALL LEDS”, this button sets all the individual lock buttons
to locked.
The main screen has a global “TURN OFF/ON ALL LEDS” pad which will disable and enable all
LEDs at once.
The main screen displays Icons for, “Light Guide Inserted”, “Alarms” and “LED on”.
Click on the “LOGOUT” button to close the GUI Control Panel. All previous data entered will be
saved upon next start up.
6.2 Set LED Triggers Screen
Figure 13 LED Trigger Screen (GUI)
The screen displays individual Trigger window sections for each LED module (e.g.: UVX, BDX,
BGX and RLX). Click the top of a wavelength section to open that selected window.
In each window section the user can change the trigger designation to OFF, Internal (indicated
by time units), External and Global. Press the “CHANGE TRIGGER” pad to select a trigger
designation. Each LED module can only have one trigger designation.
The triggers are described below:
A. The default trigger designation is set to OFF in order to allow control of the system
from the main LED screen.
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B. When in high speed triggering mode the sync signal is an acknowledgement of a
received command.
C. When the trigger is set to External: the system will accept an external signal control
to the selected LEDs. (refer to Table 8 ).
D. When the trigger is set to GLOBAL, the system will accept a trigger signal through a
single pin to control all LEDs that are set to GLOBAL. (refer to Table 8 ).
Note: When selecting External or Global trigger the LEDs will not turn on until
a trigger signal is present. The LEDs will accumulate hours while in
External or Global trigger mode. To prevent unnecessary hour
accumulation on LED modules while using the XLED1 system, users
are advised to enable these XLED1 advanced triggering modes only
during the window of time that will be used for such purposes.
Subsequent to using the advanced triggering capabilities of the XLED1
system, the user should ensure that triggering is “OFF” in the trigger
configuration screen.
E. When the trigger is set to Internal, (as indicated by “zeroes”) the user can define the
“DELAY TIME”, “ON TIME”, ‘OFF TIME” AND ‘SYNC OUT TIME”. To change the
numerical values of the times, click the up and down arrows or click the box next to
the value to open up a popup Edit Entry window where you can enter the desired
numeric value. To change the time units click the “CHANGE UNITS” pad and select a
time value unit of microseconds, milliseconds or seconds.
Note: When changing the time value units; selecting a value unit will change all
4 times associated with that LED to that unit. Each LED module can only
have one time domain unit.
F. Once all the timing parameters have been defined for each LED module, click the
“REPEAT LOOP” pad to ON (green) for automatic looping. For manual use or test
purposes ensure the “REPEAT LOOP” pad is OFF and use the “START” and “STOP”
pads to start and stop the process.
Note: Ensure that the trigger is set to OFF in order to control LEDs from the
main screen.
G. A trigger graph can be viewed (refer to section 7.5) for each LED module by clicking
on the “VIEW TRIGGER GRAPH”.
H. Once the trigger is set-up for EXTERNAL or GLOBAL, go back to the MAIN LED
screen (see below) and push the TRIGGER Button below the triggered LED. For
the LED to turn on after a trigger voltage is received, this button must be depressed.
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ADDITIONAL TRIGGER SET-UP INSTRUCTIONS
Live Cell Configuration owners should refer to section 8.0 for Live Cell Mode
Instructions
a. In internal triggering mode, the LEDs in the same time domain (a time domain is
either seconds, milliseconds or microseconds) will be automatically synchronized.
The LED period (determined by adding the Delay Time, On Time and Off Time) will
be adjusted to the longest period selected. In the example shown in graph LED A
below, the total period is 6 seconds. Then as shown in graph LED B below, the
period defined by the user is 4 seconds. In this case, an additional 2 seconds will be
added to adjust the time period to 6 seconds to match that of LED A.
b. In internal triggering mode, if the period of one LED is less than 2 times the period of
another LED in the same time domain, the system will generate additional cycles to
compensate for the longest period in the time domain. Example: In graph LED A
below the period is 6 seconds. In graph LED C the period specified was 2 seconds.
The software will automatically create three cycles to fill in the same period as LED
A. If this is not desired, the off time for LED C needs to be extended so that the LED
C total period will be equal to that of LED A. In this example change the off time
from 0 seconds to 4 seconds in order for the total period time to equal to 6 seconds.
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NOTE: The additional pulses can only be added up to a maximum which depends on
the number of channels being used in the same time domain. Typically only
12 sub pulses can be generated, if more are needed try using different time
domains to achieve the desired results.
2 sec
2 sec
2 sec
= User Specified
= System Addition
LED A Period 6 seconds
1 sec
2 sec
1 sec
LED B Period 4 seconds
1 sec
1
sec
1 sec
2 sec
LED B ext 2 sec
1
sec
1 sec
1
sec
LED C Period 2 seconds, repeated 3 times
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6.3 Trigger Graphs Screen
Figure 14 Trigger Graphs Screen (GUI)
The trigger graph screen shows all 4 LED Modules as separate graphical representations of the
triggering patterns assigned from the Set LED Trigger screen.
To return to the LED Trigger Screen touch “Return to Trigger Screen”.
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6.4 Maintenance Screen
LED MODULES
DRIVERS
DICHROICS
Figure 15 Maintenance Screen (GUI)
The Maintenance screen gives an overview of the location of the 4 individual LED modules,
drivers and dichroics.
Click on any one of the green lit Modules to retrieve the pop-up window showing the selected
Modules present attributes.
Displays LED Module wavelength range in nm.
FWHM (Full Width Half Max): e.g. depicts LED peak wavelength +/-FWHM,
the useful bandwidth of the LED.
Type: Identifies the LED Module as either Standard Power or High Power version
which is determined by the LED type.
Current: Displays the current working temperature of the LED module in °C.
Cutoff Temp: indicates the maximum specified working temperature of the LED
module in °C.
LED Used: Records the current accumulated LED module illumination hours.
Serial Number: Displays the serial number of the LED module.
Mfg ON: Displays the manufacturing date of the LED module. The remaining
touch pads are for navigating to the various screens.
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DICHROIC DATA WINDOW
Figure 17 Maintenance Screen (Dichroic Data) (GUI)
When clicking on a Dichroic module icon, a Dichroic data window will display the following
attributes:
Edge:
specified in nm (top tab of the window).
Reflect:
the wavelength range that the Dichroic will reflect, specified in nm.
Transmit:
the wavelength range the Dichroic will transmit, specified in nm.
When changing a Dichroic, the Edge value must be entered into the system. To change the
Edge value, click on the Dichroic icon to see the pop-up window, click on the top tab of the
Dichroic information window and a pop-up Dichroic Selection screen will appear. Select the new
edge value from the drop down menu and click OK to return to the maintenance screen. The
Reflect and Transmit values will be automatically populated.
If the new Dichroic is not listed in the drop down menu, refer to Section 6.5 to obtain the Edge
Wavelength, Low Reflect, High Reflect, Low Pass and High Pass numbers.
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Figure 18 Dichroic Database (GUI)
Click on the green X-Cite® Icon located on the top left corner of the maintenance screen and
click on Dichroic Database. A Dichroic Database pop-up window will appear. Fill in the number
obtained from the list below (Section 6.5) click Update Dichroic Database to save new entries.
Refer to Figure 18 .
Note: Refer to section 4.2 for LED Module and Driver replacement
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6.5 Dichroic Values
Dichroic/Edge
wavelength
409
458
495
538
605
Low
Reflect
325
350
350
350
350
High Reflect
404
450
488
528
596
Low Pass
415
467
502
547
612
High Pass
530
950
750
950
750
350
360
350
375
407
654
385
425
669
450
575
750
Special order
380
416
660
6.6 Alarm Information
Alarms
Description
Severity
Recoverable
Max Temp Exceeded
Max. LED module
Over Temperature
Protection
Critical
Yes
Min Temp Exceeded
Min. LED module
Under Temperature
Protection
Major
Yes
LED Module Fail
LED Module Failure
Critical
No
Light Guide Not
Present
Light Guide Removed
Major
Yes
Comms Error
Internal
Communication Error
Critical
No
Watchdog Timeout
Watchdog Tripped
Critical
No
NVM Fail
Non Volatile Memory
Fail
Critical
No
System Performance
System Performance
Major
Yes
Table 2 Alarm Codes (GUI)
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Figure 19 Alarm Code Screen (GUI)
Error Pop-up Screens Descriptions
Error Window
Error Description
Screen Location
"Light Guide not
inserted"
Request to turn on LED could not be
completed due to the Light Guide not
being fully inserted
All screens
"Enter Delay or Off
Time"
Request to start Pulse Generator
cycle could not be completed due to
missing delay or off time entry
Trigger screen
"To change units or
trigger, LED must be off"
Requested settings change was not
completed as the LED was on
Trigger screen
"Alarm Active on LED,
Proceed to maintenance
screen "
Request to turn on an LED could not
be completed due to an active alarm
Main screen
“Dichroic not found"
Wrong Dichroic number entered
Maintenance screen
“Function only available
on XLEDCP”
Pop-up error window appears when
trying to perform a function which is
not available via the GUI
Pop-up error window on all
screens
Table 3 Error Pop-up Screen Descriptions (GUI)
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The 8 visual alarm indicators on the Alarm Code Screen (refer to Figure 19) are indicators only
and do not function as alarm clearing buttons/pads.
The LED System is designed to detect certain conditions that may affect safe or proper
functioning. These conditions are categorized as one of two “alarm types” according to the
danger they pose to the operator, system or experiment:
1. Critical – Do not continue process, alarm shall not be clearable until “Alarm
Condition” is corrected. Contact:
http://www.excelitas.com/Pages/Support/Service-Request-Form.aspx
2. Major – operation may continue, but depending on specific alarm condition,
functionality of unit may be limited or impossible. Contact:
http://www.excelitas.com/Pages/Support/Service-Request-Form.aspx
The Alarm Information screen displays icons for the alarm codes which will light up when
triggered.
6.7 Clearing alarms
The 8 visual alarm indicators on the Alarm Code Screen (refer to Figure 19) are indicators only
and do not functional as alarm clearing buttons/pads.
The GUI system does not provide an audible alarm to alert the operator of “Light Guide not
inserted” condition.
The following visual alarms will clear automatically when the problem subsides:
- Max Temp Exceeded, Min Temp Under Limit, LED Module Fail, Light Guide
Not Present.
6.8 System Information
Figure 20 System Information (GUI)
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System Screen - Shows an overview of the complete system (GUI Control Panel and XLED1):
Errors Window:
Displays error description sent by the XLED1 detected during the power-onself-test (POST) and errors detected during operation. Refer to Table 7 for
error window descriptions.
Software Versions Window:
Displays the current software versions installed in the XLED1/Pulse
Generator/ GUI Control Panel.
Serial Number Window:
Displays the serial number of the XLED1.
LCD Settings Window:
Volume, Brightness and Timeout sliders are not functional when using the
GUI. All sliders will function and change the values if connected to the XLED1
Touch Screen Controller.
POWER ON SELF TEST:
Disabled in GUI Control Panel
ALIGN TOUCH SCREEN:
Disabled in GUI Control Panel
Error Window
Error Description
“LED over temperature”
LED module is above allowable working temperature
“LED current alarm”***
LED module is off when system is told it is on (only valid
when not in trigger mode) or LED module is on when system
is told it is off (only valid when NOT in trigger mode)
“LED under temperature”
LED module is below allowable working temperature
“LED NVM error”
LED module memory is experiencing problems
“LED ADC error”
Internal error
“LED Type Mismatch”
LED module/LED driver types are mismatched.
Table 4 Error Window Descriptions (GUI)
***Note: The alarm will not be triggered if an LED fails when the intensity is set to
under 10%. To check if an LED has failed in this case, simply move the intensity slider
to 100% to check if it is functional (WITH CAUTION)
7.
Operating the Optional Touch Screen Controller
Caution: Only connect the LGDI Touch Screen Controller to the XLED1 via rear port
as per Figure 2.
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The Touch Screen Controller is a touch screen interface to control the XLED1.
Screen Sitemap:
Log In
(refer to section 7.1)
Main LED Screen
(refer to section 7.2)
Edit LED Name
(refer to section 7.3)
Set LED Triggers
(refer to section 7.4)
Trigger Graphs
(refer to section 7.5)
Maintenance
(refer to section 7.6)
Alarm Information
(refer to section 7.7)
System Information
(refer to section 7.9)
Note: all screens have a top bar indicator:
Touch Screen Locked:
Indicates that all screens are locked, refer to section 7.2
for detailed instructions.
Light Guide Inserted:
Illuminates when the Light Guide is properly inserted.
Alarm:
Indicates a system alarm, user should navigate to the
Alarm Screen to determine the source of the alarm (refer to
section 7.7).
LED on:
Illuminated when any LED Module is on.
7.1 Log-In Screen
Figure 21 Log-In Screen (Touch Screen Controller)
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The XLED1 will automatically login as user 5 if embedded software version is 1.5 or later. The
login screen is only available by clicking logout on the main LED screen. I
Maximum Users – 5 User ID Numbers. User ID numbers must be 4 digits. The system ships
with qty.5 default ID log-in numbers 1234 / 2234 / 3234 / 4234 / 5234.
Note: User ID 5 settings will be default on XLED1 power up, if the XLED1 Touch Screen
Controller is disconnected during the next power up.
Enter the default ID 4 digit log-in number to access the system. The XLED1 will remain nonfunctional if the incorrect user ID is entered.
Upon entering User ID press ‘GO’ to navigate through all the Touch Screen Controller screens
and control the XLED1.
If logged in as a ‘Guest’ all data will not be saved after completion of session.
Note: Prior to S/W version 1.5, Ensure that users log-out of the Touch
Screen Controller before powering down or all entered data from
the latest session will not be saved. (refer to section 7.2). The
system is defaulted NOT to save any data when logged in as
‘Guest’ regardless of logging out or not. If version 1.5 or later, the
settings will be saved 5 minutes after the last setting change,
If desired the user can create a personal PIN number. Navigate to the Maintenance screen and
press the ‘CHANGE PIN’ pad.
Enter new PIN number (max 4 digits) and press ‘GO’. Re-enter the new PIN number to verify
and press ‘GO’.
A pop-up message will appear if the wrong PIN number is re-entered. Re-enter the correct new
PIN number and press ‘GO’.
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7.2 Main LED Screen
Figure 22 Main LED Screen (Touch Screen Controller)
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The main screen displays a section for each LED module, (e.g: UVX, BDX, BGX and RLX),
these can be renamed to desired fluorophores (refer to section 7.3). Tap on the desired
wavelength name/number to access the Edit LED screen. Click on the desired wavelength
name to view the wavelength range or to rename the LED. Enter a name or number and press
OK. The display will now be changed.
Each LED module section has Unlocked (grey), Locked (green), OFF (grey), and ON (green)
features. The Unlock/Lock pads, unlock or lock that individual LED module section. In lock
mode all settings (coarse and fine intensity, percentage window and wavelength window) for
that LED module are locked and cannot be changed.
Each LED module section has a coarse intensity adjustment slider and fine intensity adjustment
“UP” and “DOWN” pads. The fine intensity adjustment arrows allow intensity adjustments
from 5% to 100% in 0.1% increments. A numerical value can be entered by tapping on the %
value, which opens up a numerical keypad window.
The main screen has a global “LOCK ALL LEDS”, this button sets all the individual lock buttons
to locked.
The main screen has a global “TURN OFF/ON ALL LEDS” pad which will disable and enable all
LEDs at once.
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The main screen displays icons for “Touch Screen Locked”, “Light Guide Inserted”, “Alarms”
and “LED on”.
It is very important to use the “LOGOUT” touch pad before powering down the system. Failure to
logout before powering down will result in loss of the present settings, the system will then
default to previous settings upon the next start-up.
Ability to navigate to the “TRIGGER” screen and the “MAINTENANCE” screen .
Note: See notes in box identical to this one. Ensure that users log-out of
the Touch Screen Controller before powering down or all entered
data from the latest session will not be saved. (refer to section 7.2 ).
The system is defaulted NOT to save any data when logged in as
‘Guest’ regardless of logging out or not.
7.3 Edit LED Name Screen
Figure 23 LED Name Edit Screen (Touch Screen Controller)
The Edit screen displays a touch key pad to enable the user to rename the LED modules if
desired, e.g. – DAPI, FITC, TRITC etc. Once the name has been entered press “RETURN TO
LED SCREEN” to go back to the Main LED Screen.
The name is limited to 8 characters.
To display the centre wavelength as the wavelength name, press the “USE WAVELENGTH AS
NAME” pad.
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7.4 Set LED Triggers Screen
Figure 24 LED Trigger Screen (Touch Screen Controller)
Note: Important, when the XLED1 is in live cell mode, the internal trigger is occupied performing
that mode. This trigger screen is ineffective when operating in live cell mode. If trigger mode is
required, turn off live cell mode first. Refer to section 8 for more information.
The screen displays individual Trigger window sections for each LED module (e.g: UVX, BDX,
BGX and RLX). Touch the top of a wavelength section to open that selected window.
In each window section the user can change the trigger designation to OFF, Internal (indicated
by time units), External and Global. Press the “CHANGE TRIGGER” pad to select a trigger
designation. Each LED module can only have one trigger designation.
The triggers are described below:
A. The default trigger designation is set to OFF in order to allow control of the system
from the main LED screen.
B. When in high speed triggering mode the sync signal is an acknowledgement of a
received command.
C. When the trigger is set to External: the system will accept an external signal control
to the selected LEDs. The LED must still be turned on in the main screen, for the
external trigger to be able to turn on the LED. Refer to Table 8
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D. When the trigger is set to GLOBAL, the system will accept a trigger signal through a
single pin to control all LEDs that are set to GLOBAL. The LED must still be turned
on in the main screen, for the external trigger to be able to turn on the LED. Refer to
Table 8
Note: When selecting External or Global trigger the LEDs will not turn on until
a trigger signal is present. The LEDs will accumulate hours while in
External or Global trigger mode. To prevent unnecessary hour
accumulation on LED modules while using the XLED1 system, users
are advised to enable these XLED1 advanced triggering modes only
during the window of time that will be used for such purposes.
Subsequent to using the advanced triggering capabilities of the XLED1
system, the user should ensure that triggering is “OFF” in the trigger
configuration screen.
E. When the trigger is set to Internal, (as indicated by “zeroes”) the user can define the
“DELAY TIME”, “ON TIME”, ‘OFF TIME” AND ‘SYNC OUT TIME”. To change the
numerical values of the times, press the up and down arrows or touch the box next to
the value to open up a key pad screen where you can enter the desired numeric
value. To change the time units press the “CHANGE UNITS” pad and select a time
value unit of microseconds, milliseconds or seconds.
Note: When changing the time value units; selecting a value unit will change all
4 times associated with that LED to that unit. Each LED module can only
have one time domain unit.
F. Once all the timing parameters have been defined for each LED module, press the
“REPEAT LOOP” pad to ON (green) for automatic looping. For manual use or test
purposes ensure the “REPEAT LOOP” pad is OFF and use the “START” and “STOP”
pads to start and stop the process.
Note: Ensure that the trigger is set to OFF in order to control LEDs from the
main screen.
G. A trigger graph can be viewed (refer to section 7.5) for each LED module by touching
the “VIEW TRIGGER GRAPH”.
H. Once the trigger is set-up for EXTERNAL or GLOBAL, go back to the MAIN LED
screen (see below) and push the TRIGGER Button below the triggered LED. For
the LED to turn on after a trigger voltage is received, this button must be depressed.
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ADDITIONAL TRIGGER SET-UP INSTRUCTIONS
Live Cell Configuration owners should refer to section 8.0 for Live Cell Mode
Instructions
a. In internal triggering mode, the LEDs in the same time domain (a time domain is
either in seconds, milliseconds or microseconds) will be automatically synchronized.
The LED period (determined by adding the Delay Time, On Time and Off Time) will
be adjusted to the longest period selected. In the example shown in graph LED A
below, the total period is 6 seconds. Then as shown in graph LED B below, the
period defined by the user is 4 seconds. In this case, an additional 2 seconds will be
added to adjust the time period to 6 seconds to match that of LED A.
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b. In internal triggering mode, if the period of one LED is less than 2 times the
period of another LED in the same time domain, the system will generate
additional cycles to compensate for the longest period in the time domain.
Example: In graph LED A below the period is 6 seconds. In graph LED C the
period specified was 2 seconds. The software will automatically create three
cycles to fill in the same period as LED A. If this is not desired, the off time for
LED C needs to be extended so that the LED C total period will be equal to that
of LED A. In this example change the off time from 0 seconds to 4 seconds in
order for the total period time to equal to 6 seconds.
NOTE: The additional pulses can only be added up to a maximum which depends on
the number of channels being used in the same time domain. Typically only
12 sub pulses can be generated, if more are needed try using different time
domains to achieve the desired results.
2 sec
2 sec
2 sec
= User Specified
= System Addition
LED A Period 6 seconds
1 sec
2 sec
1 sec
LED B Period 4 seconds
1 sec
1
sec
1 sec
2 sec
LED B ext 2 sec
1
sec
1 sec
1
sec
LED C Period 2 seconds, repeated 3 times
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7.5 Trigger Graphs Screen
Figure 25 Trigger Graphs Screen (Touch Screen Controller)
The trigger graph screen shows all 4 LED Modules as separate graphical representations of the
triggering patterns assigned from the Set LED Trigger screen.
To return to the LED Trigger Screen touch “Return to Trigger Screen”.
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7.6 Maintenance Screen
LED MODULES
DRIVERS
DICHROICS
Figure 26 Maintenance Screen (Touch Screen Controller)
The maintenance screen gives an overview of the locations of the 4 individual LED Modules,
Drivers and Dichroics.
Touch any one of the green lit Modules to retrieve the pop-up window showing the selected
Modules present attributes.
Displays LED Module wavelength range in nm.
FWHM (Full Width Half Max): e.g. depicts LED peak wavelength +/-FWHM,
the useful bandwidth of the LED.
Type: Identifies the LED Module as either Standard Power or High Power version
which is determined by the LED type.
Current: displays the current working temperature of the LED module in °C.
Cutoff Temp: indicates the maximum specified working temperature of the LED
module in °C.
LED Used: Records the current accumulated LED module illumination hours.
Serial Number: displays the serial number of the LED module.
Mfg ON: displays the manufacturing date of the LED module. The remaining
touch pads are for navigating to the various screens.
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DICHROIC DATA WINDOW
Figure 27 Maintenance Screen (Dichroic Data) (Touch Screen Controller)
When touching a Dichroic icon, a Dichroic pop-up window will display the following attributes:
Edge:
specified in nm (top tab of the window).
Reflect:
the wavelength range that the Dichroic will reflect, specified in nm.
Transmit:
the wavelength range the Dichroic will transmit, specified in nm.
When changing a Dichroic, the Edge value must be manually entered into the system. To
change the Edge value, touch on the Dichroic icon to see the pop-up window, touch the top tab
of the Dichroic information window and an edit screen will appear. Type in the new Edge value
and press “GO” to return to the maintenance screen. Refer to section 11 for Dichroic
replacement.
For new Dichroics, the reflect and transmit values will not be automatically updated, refer to
Section 6.5 for these values. The data cannot be entered into the Touch Screen Controller
manually. The system will light up the new Dichroic in red. This means the data is not available
but the system will still be functional. To update the values, use the GUI on your PC, refer to
sections 0 to 0.
Note: Refer to section 4.2 for LED Module and Driver replacement
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7.7 Alarm Information
Alarms
Description
Severity
Recoverable
Max Temp Exceeded
Max. LED Module Over Temperature
Protection
Critical
Yes
Min Temp Exceeded
Min. LED Module Under Temperature
Protection
Major
Yes
LED Module Fail
LED Module Failure
Critical
No
Light Guide Not
Present
Light Guide Removed or not fully
inserted
Major
Yes
Comms Error
Internal Communication Error
Critical
No
Watchdog Timeout
Watchdog Tripped
Critical
No
NVM Fail
Non Volatile Memory Fail
Critical
No
System Performance
System Performance
Major
Yes
Table 5 Alarm Codes (Touch Screen Controller)
Figure 28 Alarm Code Screen (Touch Screen Controller)
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Error Pop-up Screens Descriptions
*
*
Error Window
Error Description
Screen Location
"Light Guide not
inserted"
Request to turn on LED could not
be completed due to Light Guide
not fully inserted
All screens
"Enter Delay or Off
Time"
Request to start Pulse Generator
cycle could not be completed due
to missing delay or off time entry
Trigger screen
"To change units or
trigger, LED must be
off "
Requested settings change was not
completed as the LED was on
Trigger screen
"User ID is incorrect"
Entered incorrect User ID
Login screen
“Retry or enter as
Guest"
Entered incorrect User ID
Login screen
"Number is out of
range"
The number value entered is out of
range
Number input pad
"Alarm Active on
LED, Proceed to
maintenance screen "
Request to turn on an LED could
not be completed due to an active
alarm
Main screen
“Dichroic not found"
Wrong Dichroic number entered
Maintenance screen
"PIN already in use"
If PIN is the same as another user’s
During PIN update wizard
"Please choose
another"
If PIN is the same as another user’s
During PIN update wizard
“PINs do not match!"
If the second PIN does not match
the first
During PIN update wizard
"Guest cannot
change PIN!"
If change PIN button is pressed and
the current user is Guest
Maintenance screen
3 audible beeps are only generated when the Touch Screen Controller is connected.
Table 6 Error Pop-up Screen Descriptions (Touch Screen Controller)
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The 8 visual alarm indicators on the Alarm Screen (refer to Figure 28) are indicators only and do
not function as alarm clearing buttons/pads.
The LED System is designed to detect certain conditions that may affect safe or proper
functioning. These conditions are categorized as one of two “alarm types” according to the
danger they pose to the operator, system or experiment:
1. Critical – Do not continue process, alarm shall not be clearable until “Alarm
Condition” is corrected. Contact: http://www.excelitas.com/Pages/Support/ServiceRequest-Form.aspx
2. Major – operation may continue, but depending on specific alarm condition,
functionality of unit may be limited or impossible. Contact:
http://www.excelitas.com/Pages/Support/Service-Request-Form.aspx
The Alarm Information screen displays touch pads for the alarm codes which will light up and
generate an audible signal when triggered.
7.8 Clearing alarms
The 8 visual alarm indicators on the Alarm Screen (refer to Figure 28) are indicators only and do
not functional as alarm clearing buttons/pads.
The system provides an audible alarm to alert the operator of “Light Guide not inserted”
condition only
The audible alarm cannot be silenced and will generate 3 audible beeps only.
The following visual alarms will clear automatically when the problem subsides or is corrected:
- Max Temp Exceeded, Min Temp Under Limit, LED Module Fail, Light Guide
Not Present.
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7.9 System Information
Figure 29 System Information (Touch Screen Controller)
System Information screen: Shows an overview of the complete system (Touch Screen
Controller and XLED1);
Errors Window:
Displays error description sent by the XLED1 detected during the power-onself-test (POST) and errors detected during operation. Refer to Table 7 for
error window descriptions.
Software Versions Window:
Displays the current software versions installed in the XLED1/Pulse
Generator/ Touch Screen Controller.
Serial Number Window:
Displays the serial number of the XLED1.
LCD Settings Window:
This screen allows adjustments to the Volume (alarm), Touch Screen
Controller screen Brightness and the Touch Screen Controller screen saver
Timeout. Drag the sliders up or down for desired settings.
To disable the “Timeout” (sleep) mode set slider to “0”.
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The screen will go blank after timeout lapses. Touch the screen anywhere to
return to present screen
POWER ON SELF TEST:
Press to start the Power On Self Test. The system will commence with a
Power-On-Self-Test (POST). All outputs will be inactive and all inputs will be
unresponsive during the POST cycle. In the event of a POST error, the
system will report the error and either allow the user to proceed or halt,
depending on the error condition. Refer to section 7.7 for error codes.
ALIGN TOUCH SCREEN:
Press this pad to calibrate the touch screen accuracy.
Upon pressing the pad follow the instructions on the screen, there is a
timeout of 10 seconds between each test. If time lapses the Touch Screen
Controller will return to the “System Information Screen”. Press this pad again
to calibrate.
Error Window
Error Description
“LED over temperature”
LED Module is above allowable working temperature
“LED current off alarm”***
LED Module is off when system is told it is on (only valid
when not in trigger mode)
“LED under temperature”
LED Module is below allowable working temperature
“LED current on alarm”
LED Module is on when system is told it is off (only valid
when NOT in trigger mode)
“LED NVM error”
LED Module memory is experiencing problems
“LED ADC error”
Internal error
“LED Type Mismatch”
LED Module/LED driver types are mismatched
Table 7 Error Window Descriptions (Touch Screen Controller)
***Note: The alarm will not be triggered if an LED fails when the intensity is set to
under 10%. To check if an LED has failed in this case, simply move the intensity slider
to 100% to check if it is functional (WITH CAUTION)
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8.
Live Cell Mode (on Live Cell Configuration units)
Live Cell Mode is a feature embedded in XLED1 Live Cell Configuration units (includes modules
BLX, BDX, BGX and GYX). This feature allows any enabled LEDs (together or individually) to
switch between pulsed and continuous mode of operation through the LED ON/OFF button on
the front of the unit.
NOTE: It is NOT recommended to have both the touch screen controller and GUI connected to
the XLED1 at the same time.
8.1 Enabling Live Cell Mode
Hold down the LED ON/OFF button for at least 5 seconds (until light guide indicator light turns
orange).
Once enabled, pressing the LED ON/OFF button will cause all 4 LEDs to turn on at 100% power
and pulse at the default duty cycle of 50% with a period of 2ms (i.e., 1ms ON, 1ms OFF). To
turn off the LEDs, briefly press and release the LED ON/OFF button.
NOTE: The Live Cell Mode is essentially an internal trigger, and the control panel will appear as
though a trigger is ON. Choosing the LEDs that are enabled along with their intensities can be
done through the touch screen controller or the GUI. To ensure that an LED is disabled upon
pressing the LIVE CELL MODE button on the front of the unit, the intensity of the LED must be
set to zero on the slider in the control panel.
If LEDs are turned on through the controller or GUI, only those LEDs that are ON will pulse at the
default duty cycle of 1ms, at the intensity set on the LED slider. To disable Live Cell Mode the
front panel LED ON/OFF button must be depressed until Light Guide indicator light turns green
(hold on/off button down for at least 5 seconds).
LIGHT GUIDE INDICATOR
MASTER POWER
ON/OFF SWITCH
LED ON/OFF
BUTTON
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8.2 Changing duty cycle in Live Cell Mode
The duty cycle may be changed using the XLED1 touch screen controller OR the GUI but NOT
both and NOT while the LEDs are on.
The Trigger MUST be stopped in the TRIGGER control screen before changing duty cycle
Using XLED1 touch screen controller:
8.2.1.1
Once triggers are stopped, make the change required to the pulse duration (by
adjusting ON time and OFF time).
8.2.1.2 To save these setting for future use, the user must use the LOG OUT button.
8.2.1.3 When you log in again, and enable Live Cell Mode (through the front panel
button), the last saved settings (chosen LEDs, intensity and pulse duration) will
be used for pulsing
8.2.1.4 NOTE: If it is desired to save the changes when the XLED1 touch screen
controller is not connected, login as user number 5. If touch screen controller is
plugged in, the last change made (before logging out, settings are only saved on
logout) will take effect.
Using the GUI:
8.2.1.5 Changes can be made to duty cycle and intensity as described above
8.2.1.6
When LIVE CELL mode is enabled, these changed will apply when the LED(s)
are turned ON.
8.2.1.7 When logging out of the GUI make sure to “SAVE SETTINGS”. Note: This option
only appears if the XLED1 touch screen controller is not connected.
8.2.1.8 When the unit is turned on in the future and live cell mode is enabled, the last
trigger screen settings and LED intensities are applied.
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9.
External Control
9.1 External Trigger Interface
SYNC OUT PORT
TRIGGER INPUT PORT
Figure 30 Terminal Block Connectors
The rear panel of the XLED1 (refer to Figure 30) has two HD15 connectors. One 15-pin HD15
connector for input triggers (refer to Table 8 ) and one 15-pin HD15 connector for sync
output(refer to Table 9 ).
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Table 8 External Trigger Pin Assignments
PIN #
Signal name
Description
Location
1
Port 1 trigger +
Port 1 trigger input : High = LED on;
Low = LED off
Port 1 (red BNC cable)
2
Port 2 trigger +
Port 2 trigger input : High = LED on;
Low = LED off
Port 2 (green BNC cable)
3
Port 3 trigger +
Port 3 trigger input : High = LED on;
Low = LED off
Port 3 (blue BNC cable)
4
n/c
Not Connected
5
n/c
Not Connected
6
Port 1 trigger -
Ground
Port 1 (red BNC cable)
7
Port 2 trigger -
Ground
Port 2 (green BNC cable)
8
Port 3 trigger -
Ground
Port 3 (blue BNC cable)
9
n/c
Not Connected
10
n/c
Not Connected
11
Port 4/Global trigger -
Ground
12
n/c
Not Connected
13
Port 4 trigger +
Port 4 trigger input : High = LED on;
Low = LED off
Port 4 (white BNC cable)
14
Global trigger +
Global trigger input : High = LED on;
Low = LED off
Global (black BNC cable)
15
n/c
Not Connected
Port 4 (white+black BNC cable)
Table 9 External Sync Out Pin Assignments (OUTPUT 5.5V, 8mA max)
PIN #
Signal name
Description
Location
1
Port 1 sync +
Port 1 Sync out : High = LED on; Low = LED
off
Port 1 (red BNC cable)
2
Port 2 sync +
Port 2 Sync out : High = LED on; Low = LED
off
Port 2 (green BNC cable)
3
Port 3 sync +
Port 3 Sync out : High = LED on; Low = LED
off
Port 3 (blue BNC cable)
4
n/c
Not Connected
5
n/c
Not Connected
6
Port 1 sync -
Ground
Port 1 (red BNC cable)
7
Port 2 sync -
Ground
Port 2 (green BNC cable)
8
Port 3 sync -
Ground
Port 3 (blue BNC cable)
9
n/c
Not Connected
10
n/c
Not Connected
11
Port 4 sync -
Ground
12
n/c
Not Connected
Port 4 (white BNC cable)
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PIN #
Signal name
Description
Location
13
Port 4 sync +
Port 4 Sync out : High = LED on; Low = LED
off
Port 4 (white BNC cable)
14
n/c
Not Connected
15
n/c
Not Connected
t2
Trigger
t1
t3
Sync
t1 – pre-sync time
t2 – sync delay time
t3 – post-sync time
9.2 Trigger Input/Sync Output Signals Requirements
Trigger Input Signal Requirements
o
o
o
Low-level input : 0 ~ +0.8VDC
High-level input: +4.5 ~ +5.5VDC
Maximum input current: 6.5mA
Sync Output Signal Requirements:
o
o
o
Low-level output : 0 ~ +0.8VDC
High-level output : +4.5 ~ +5.5 VDC
Maximum output current: 100uA
9.3 I/O Signal Descriptions
External Trigger Input
1
1
1
5V signal applied to Port 1 trigger +, this will turn on LED Module 1, when LED Module 1 is
in external trigger mode.
0V signal applied to Port 1 trigger +, this will turn off LED Module 1, when LED Module 1 is
in external trigger mode.
LED Module 1 is not necessarily the lowest wavelength installed refer to figures 4, 22, 23 for configurations.
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5V signal applied to Port 2 trigger +, this will turn on LED Module 2, when LED Module 2 is
in external trigger mode.
0V signal applied to Port 2 trigger +, this will turn off LED Module 2, when LED Module 2 is
in external trigger mode.
5V signal applied to Port 3 trigger +, this will turn on LED Module 3, when LED Module 3 is
in external trigger mode.
0V signal applied to Port 3 trigger +, this will turn off LED Module 3, when LED Module 3 is
in external trigger mode.
5V signal applied to Port 4 trigger +, this will turn on LED Module 4, when LED Module 4 is
in external trigger mode.
0V signal applied to Port 4 trigger +, this will turn off LED Module 4, when LED Module 4 is
in external trigger mode.
5V signal applied to Global trigger +, this will turn on all LED Modules which are in global
trigger mode.
0V signal applied to Global trigger +, this will turn off all LED Modules which are in global
trigger mode.
External Sync Output
2
2
A 5V signal will be produced on Port 2 sync +, when LED Module 2 has been triggered.
A 0V signal will be produced on Port 2 sync +, when LED Module 2 has not been triggered.
A 5V signal will be produced on Port 3 sync +, when LED Module 3 has been triggered.
A 0V signal will be produced on Port 3 sync +, when LED Module 3 has not been triggered.
A 5V signal will be produced on Port 4 sync +, when LED Module 4 has been triggered.
A 0V signal will be produced on Port 4 sync +, when LED Module 4 has not been triggered.
2
A 5V signal will be produced on Port 1 sync +, when LED Module 1 has been triggered.
A 0V signal will be produced on Port 1 sync +, when LED Module 1 has not been
triggered.
LED Module 1 is not necessarily the lowest wavelength installed refer to figures 4, 22, 23 for configurations.
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10. LED Module and Driver Replacement
CAUTION
To protect the LED Module, Driver and system from electrostatic discharge (ESD)
related damage, which can disable the system, cause latent defects or permanent
damage, or require repair by service technicians, please take the following ESD
prevention steps:
Ensure the unit is unplugged
Ensure you are properly grounded, ideally by using an ESD grounding strap attached
to an ESD protective surface.
Refer to section 2.3 for principles of ESD and additional ESD precautions.
Note: Ensure that users log-out of the Touch Screen Controller before
powering down or all entered data from the latest session will not be
saved. (refer to section 7.2). The system is defaulted NOT to save any
data when using the system logged in as ‘Guest’ regardless of logging
out or not.
Tools Required:
Locking Hub Key (supplied)
#2 Philips Head Screwdriver (not supplied)
Torque tool (not supplied)
LED Modules, Drivers and Dichroics are shipped in protective ESD bags.
LED Modules are shipped with a protective lens cap covering the LED lens.
Ensure the system is OFF and the AC power cord is unplugged from the power source. Allow
system to cool for 5 minutes before removing the housing.
Using the locking hub key supplied, rotate the green locking hubs 90 degrees and remove the
locking hubs. Holding the XLED1 housing at the back, slide the XLED1 housing up and back
(away from the fascia) for removal. The locking hub key can be stored on the locking hub key
hanger, located on the back panel of XLED1.
Figure 31 Locking Hub
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Replacement LED Modules are shipped with their unique Drivers. Both components must be
replaced at the same time. Refer to Figure 32.
Note: Remove the corresponding Dichroic before removing the LED module and
driver to prevent accidental contact with the Dichroic. Refer to section 11.
To remove the LED Module and Driver unscrew the Philips-Head screws and remove Module
and Driver.
Caution: Do not touch the LED lens surface. Skin oils and scratches can cause the
optical output to decrease.
Firstly, remove the protective LED lens cap if present on the new LED module. To replace the
LED Module and Driver align the LED Module and Driver male pins with the female socket
connection and gently push down into place. Tighten the Philips-Head screws until a positive
force is felt. DO NOT OVER TIGHTEN THE SCREWS. (If using a calibrated torque-meter,
Excelitas Technologies recommends a torque value of 5.7 lb-in/± 0.3 lb-in.)
Replace the Dichroic (refer to section 11).
If there is a mismatch installation of either standard powered or high powered Drivers, the
corresponding LED will not operate.
Replace the XLED1 housing ensuring a snug fit. Insert the locking hubs and using locking hub
key supplied, rotate the green locking hubs to the locked position. Return the locking hub key to
the locking hub key hanger, located at the rear of the system. The system will not allow the main
power switch to function if the unit cover is not replaced properly.
Note: For assistance in determining the appropriate positions for Modules and
Dichroics visit:
http://www.excelitas.com/Pages/Product/X-Cite-LED-ModuleConfigurator.aspx
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Figure 32 LED Module and Driver Replacement
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11. Dichroic Replacement
CAUTION
To protect the LED Module, Driver and system from electrostatic discharge (ESD)
related damage, which can disable the system, cause latent defects or permanent
damage, or require repair by service technicians, please take the following ESD
prevention steps:
Ensure the unit is unplugged
Ensure you are properly grounded, ideally by using an ESD grounding strap attached
to an ESD protective surface.
Refer to section 2.3 for principles of ESD and additional ESD precautions
Note: Ensure the users log-out of the Touch Screen Controller before
powering down or all entered data from the latest session will not be
saved. (refer to section 7.2). The system is defaulted NOT to save any
data when using the system logged in as ‘Guest’ regardless of logging
out or not.
Tools Required:
Locking Hub Key (supplied)
DICHROIC OPTICAL
WINDOW
Caution:
The Dichroic optical window operational life can be significantly shortened if it is
handled incorrectly. Do not touch the window surface. Skin oils and scratches can
cause the window to fail prematurely. Refer to section 12.3 for handling and cleaning
instructions.
Ensure the system is OFF and the AC power cord is unplugged from the power source.
Using the locking hub key supplied, rotate the green locking hubs and remove the XLED1
housing, refer to section 0.
Note: When replacing the Dichroic remove and replace one at a time.
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Remove the plastic locking screw and gently pull out the module, avoid touching the Dichroic
optical window with fingers, microscope immersion oil, or any abrasive object. Refer to section
12.3 for handling and cleaning instructions. Note the orientation of the Dichroic when removing,
there is a notched cut-out on the top plate which allows the Dichroic’s orientation key to pass
through, subsequently preventing the Dichroic from being replaced incorrectly. Refer to Figure
33.
Replace the Dichroic by inserting through the slot and lightly press down until a positive force is
felt. Hand tighten the locking screw, do not use mechanical tooling such as screw drivers, pliers
etc.
If the Dichroic is inserted incorrectly, the module’s orientation key (located on the module
frame), will not pass through the cut-out on the top plate resulting in an unseated Dichroic.
Replace the XLED1 housing ensuring a snug fit. Using the locking hub key supplied, rotate the
green locking hubs to the locked position. Return the locking hub key to the locking hub key
hanger, located at the rear of the system. The system will not allow the main power switch to
function if the housing is not replaced properly.
When changing a Dichroic the Edge value must be manually entered into the system. Refer to
section 7.6 to change the Edge value, touch on the Dichroic to see the pop-up window, touch
the top tab of the window and an edit screen will appear. Type in the new Edge value and press
“GO” to return to the maintenance screen. The Edge value is printed on the Dichroic.
The Reflect and Transmit values in the pop-up window will be automatically populated if using
the GUI. Refer to section 6.4.
LOCKING SCREW
ORIENTATION KEY
DICHROIC OPTICAL
WINDOW
ORIENTATION CUT-OUT
DICHROIC
MODULE
Figure 33 Dichroic Replacement
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12. Routine Care and Maintenance
To protect the system from electrostatic discharge (ESD) related damage, which can
disable the system, cause latent defects or permanent damage, or require repair by
service technicians, please take the following ESD prevention steps:
Ensure the unit is unplugged
Ensure you are properly grounded, ideally by using an ESD grounding strap attached
to an ESD protective surface.
Refer to section 2.3 for principles of ESD and additional ESD precautions
Caution: Routine maintenance should only be completed by qualified technical
personnel to avoid risk of injury to the end user.
Warning: Use UV eye and skin protection during servicing if access to the UV LED
Modules is required during system operation.
12.1 General
Operate the unit in a well ventilated area with at least 50mm clearance on all sides to allow
adequate space for the air vents located on the bottom of the unit.
Always connect the XLED1 external power module AC supply cord to a grounded outlet
(earthed mains) only.
When necessary, clean the aperture of the Light Guide using an optical cleaning solution. Refer
to section 12.5.
12.2 Cleaning Procedure, XLED1 & Touch Screen Controller:
Ensure the XLED1 master power switch is in the off position and the AC power cable is
unplugged.
Use a mild soap solution as the cleaning media.
Dampen a clean lint free cloth, tissue or swab with the mild soap solution and wipe the exterior
of the housing. Avoid excessive moisture when wiping in the air vent areas.
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Using a new dampened tissue (or pre-saturated tissue), clean the Touch Screen Controller
display by gently wiping the surface with one continuous stroke in one direction.
Note: Never apply a dirty tissue or swab to the display of the Touch Screen
Controller
Inspect the display to verify no residue is present.
Note: Regular cleaning of the display is recommended for optimal performance.
CAUTION: Do not use any alcohol based or acetone based solutions for cleaning
the XLED1 or the Touch Screen Controller.
12.3 Handling and Cleaning of the LED Lens Assembly
Cleaning of the lens assembly is not generally required. However, if any visible
contamination or finger prints appear in the lens surface, the contaminants may cause
reduced light output. Light output degradation may also result from scratches, digs and
other damage on the lens surface. Refrain from touching the optical window with any
foreign object which could scratch the optical window.
Caution: Do not touch the LED lens surface. Skin oils and scratches can cause the optical
output to decrease.
Cleaning Procedure for LED Lens Assembly
Improper handling and cleaning practices can damage lens surface which are
utilized in this assembly. Proper handling and cleaning technique should be adhered
to. Below are the recommended procedures for cleaning of the lens assembly.
Handling:
Always handle the lens assembly by the metal frame; never touch the lens surface
with your fingertips. The moisture or oils on your fingertips can damage the lens
surface. Fingerprints left on a lens surface for an extended period of time can
become a permanent stain. Even if you are wearing gloves, avoid touching the lens
surface.
Never handle the lens assembly with metal implements or tweezers.
To store lens assemblies, wrap them individually in a clean, ESD safe bag and store
in a safe, dry place.
Never store multiple unwrapped lens assemblies in the same bag, as they may
become damaged if they come in contact with each other.
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Cleaning Materials:
The following materials are recommended for cleaning the lens assembly.
Rubber Bulb Dust Blower (non-pressurized).
Lint-free lens tissue, lint-free cotton swabs, lint or powder-free gloves or finger cots.
An organic solvent, such as reagent-grade isopropyl alcohol or lens cleaning
solution.
Note: Avoid using any type of pressurized air/nitrogen systems for cleaning as there
is a possibility of leaving an oil or chemical film on the lens surface.
Lens Assembly Cleaning Procedure:
Wear powder free latex gloves or finger cots to protect hands from solvents and
prevent lens from contamination.
Blow off dirt and dust on lens surface with rubber dust blower.
Use a lens tissue or a lint-free cotton swab. Saturate the lens tissue or swab with
organic solvent.
CAUTION: Before using any solvent, consult the manufacturer's Materials Safety Data
Sheets (MSDS) and your internal Health and Safety Advisor for proper
handling and storage.
Note: Never re-install the lens assembly before the solvent has evaporated.
12.4 Handling and Cleaning of the Dichroic
Cleaning of the Dichroic is not generally required. However, if any visible contamination or
finger prints appear in the Dichroic optical window, the contaminants may cause reduced
light output and further damage to the system. Light output degradation may also result
from scratches, digs and other coating damage on the Dichroic window surface. Refrain
from touching the optical surface with any foreign object which could scratch the optical
window.
Cleaning Procedure for Dichroic
The Dichroic includes a coated substrate (Dichroic optical window) and module
frame assembly. Improper handling and cleaning practices can damage surfaces or
coatings which are utilized in this assembly. Note that damage to the surface of the
Dichroic coating can degrade the light output performance. Proper handling and
cleaning technique should be adhered to. Below are the recommended procedures
for cleaning of the Dichroic.
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Handling:
Always handle the Dichroic by the metal frame; never touch the Dichroic optical
window assembly with your fingertips. The moisture or oils on your fingertips can
damage the coating of the glass in the optical window. Fingerprints left on an optical
surface for an extended period of time can become a permanent stain. Even if you
are wearing gloves, avoid touching the optical surface.
Never handle the Dichroic optical window with metal implements or tweezers.
To store Dichroics, wrap them individually in a clean, protective ESD bag and store
in a safe, dry place.
Never store multiple unwrapped Dichroics in the same bag, as they may become
damaged if they come in contact with each other.
Cleaning Materials:
The following materials are recommended for cleaning the Dichroic.
Rubber Bulb Dust Blower (non-pressurized).
Lint-free lens tissue, lint-free cotton swabs, lint or powder-free gloves or finger cots.
An organic solvent, such as reagent-grade isopropyl alcohol or lens cleaning
solution.
Note: Avoid using any type of pressurized air/nitrogen systems for cleaning as there
is a possibility of leaving an oily or chemical film on the Dichroic surface.
Dichroic Optical Window Cleaning Procedure:
Wear powder free latex gloves or finger cots to protect hands from solvents and
prevent window from contamination.
Blow off dirt and dust on glass surface with rubber dust blower.
Use a lens tissue or a lint-free cotton swab. Saturate the lens tissue or swab with
organic solvent.
CAUTION: Before using any solvent, consult the manufacturer's Materials Safety Data
Sheets (MSDS) and your internal Health and Safety Advisor for proper
handling and storage.
Note: Never re-install the Dichroic before the solvent has evaporated
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12.5 Tips to Prevent Premature Degradation of Light Guides
Liquid Light Guides have a typical useful life of 2-3 years when installed and handled properly.
The formation of bubbles is one of the most common reasons for a Light Guide to degrade
prematurely and result in a reduction in illumination intensity. Bubbles form without warning,
usually due to overheating and/or mechanical stress to the Light Guide. Below are some simple
tips to avoid overheating and stressing the Light Guide.
The Light Guide has a minimum bend radius of 1.6 inches (40.0mm). Bending or coiling the
Light Guide tighter than this radius will result in permanent damage to the Light Guide.
Make sure the Light Guide is cooled properly during use, and prevent overheating:
a. Always fully insert the Light Guide into XLED1: this ensures contact with a heat
sink to conduct heat away from the Light Guide.
b. Never obstruct the air vents on XLED1. Vents are located at the rear and
underside of the unit.
c. Do not remove the rubber feet on XLED1 or otherwise reduce/block the space
between the bottom of the unit and bench top. This may compromise airflow
through the unit.
d. Ensure that the air being used to ventilate XLED1 is approximately “room
temperature” (e.g. do not place the X-Cite® unit on top of another heat-producing
instrument).
e. If a heated environmental chamber (or incubator) is being used for live cell
imaging, make sure that XLED1 and the Light Guide are located outside of the
chamber (or incubator).
Do not expose the Light Guide to extreme temperatures (above 35°C, below -5°C) for extended
periods of time during use, transport or storage; this may cause degradation of the seals and
allow air bubbles to form in the liquid.
Never kink, bend, crush, or stretch the Light Guide; this type of mechanical stress may cause
bubbles to form in the liquid and/or damage to the outer sheath.
a. Always allow adequate clearance between the Light Guide port of XLED1 and
other objects to prevent excessive bending.
b. Place XLED1 close enough to the microscope so that there is some slack in the
Light Guide and no sharp bends.
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Never leave an end cap on the output end of the Light Guide when the other end is connected
to XLED1; if the unit is turned on in this condition, the cap will overheat, melt and/or
permanently discolour the quartz end of the Light Guide.
While XLED1 is on but not in actual use (i.e. during sample preparation, or between time lapse
time points) it is recommended to disable the LED source by depressing (turning off) the LED
“ON/OFF” button (refer to Figure 1) or through the GUI Control Panel (refer to section 6.1) or
through the Touch Screen Controller, (refer to section 7.2) this reduces unnecessary photon
load on the liquid Light Guide. (Using only the shutter/stop in the microscope itself protects the
specimen, but energy is still passing through the Light Guide).
What does a bubble look like?
Depending on the size and location in the Light Guide, a bubble may or may not be obvious. To
check for bubbles:
Disconnect the LLG from the XLED1 and microscope adapter.
Hold one end towards a bright window or overhead room light - DO NOT use the XLED1 or any
other focused light source for this test!
Look at the quartz at the other end of the LLG.
a. Bubble-free: quartz end will appear as a bright, solid circle; you may also be
able to see a thin circular outline at the quartz/liquid interface.
b. Bubbles at/near the quartz end: appear as dark spots, as small as 0.5mm in
diameter or even as larger more defined spheres.
c. Bubbles in the middle of the Light Guide: may not be well-defined spots, but
will appear as dark shadows.
d. In extreme cases, where the bubble is blocking the entire diameter of the
Light Guide, no light will come through, even when pointing the distal end at a
light source.
100%
11.3%
5.2%
Appearance and % output of Light Guides
with bubbles relative to an LLG without bubbles (100%).
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Can a bubbled Light Guide recover?
Light Guides with small bubbles can sometimes recover. Disconnect the Light Guide from the
XLED1, and leave the Light Guide undisturbed on a shelf for 1-2 weeks at temperatures
between 2°C – 20°C. For this to be effective, it is important to catch the bubble when it is small.
When should Light Guides be replaced?
It is usually time to replace a Light Guide when:
a. Dark or uneven areas become visible in the field of view (a bubble is blocking
part of the light).
b. A section of the Light Guide becomes noticeably warmer than the rest of the
guide (a bubble is blocking transmission of light, forcing the Light Guide to
absorb the energy).
c. It is 2-3 years old OR has been in use for 4000-6000 hours.
13. Troubleshooting Symptom/Possible Cause
CAUTION: Service to be completed by qualified technical personnel only!
If the XLED1 fails to POWER up or function properly, use the following checklist to
eliminate the most common causes of problems. Check for the following:
1. The power supply cord is securely connected to a grounded (earthed mains socket)
functional 3-pin outlet.
2. The power supply cord is securely connected into the 3-pin outlet on the rear of the
controller unit.
3. Ensure the housing is installed correctly.
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If the light intensity is too low, check for the following:
1. Ensure Power is off. Verify that the emitting end of the Light Guide has been properly
cleaned.
2. Verify that the intensity level of the LED Modules are set correctly, (GUI Control Panel
– Main LED Screen) or (Touch Screen Controller Main LED screen) and the LEDs
have been enabled and triggers are off.
3. Ensure Dichroic Modules are installed correctly.
4. Check the Dichroic Edge value matches the actual value printed on the Dichroic:
a. Turn off system (ensuring to properly log out).
b. Open the housing and remove each Dichroic module one at a time taking note of
the Dichroic Edge value printed on the Dichroic module.
c. Install the housing.
d. Start up system and navigate to the maintenance screen. Select each Dichroic
green lit module and compare pop-up screen edge value to the actual printed
value.
If the optional Touch Screen Controller display does not illuminate:
1. Verify that the Touch Screen Controller is plugged into the XLED1 and the XLED1
turned on.
2. Verify the screen brightness is turned up (section 7.9 Touch Screen Controller System
Information Screen).
3. Verify screen Time-out mode setting (section 7.9 Touch Screen Controller System
Information Screen).
Touch Control/Screen Accuracy:
1. If the touch control is lagging, navigate to the System Information Screen to run a
screen calibration for screen accuracy, follow these steps:
LOG IN
Main LED Screen
Press the Maintenance Screen pad
Press the System Pad
Press the Align Touch Screen Pad (refer to section 7.9)
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14. Technical Specifications
LED Modules:
Central Wavelength/FWHM:
Refer to http://www.excelitas.com/Pages/Product/X-Cite-LEDModule-Configurator.aspx for available wavelengths
Dimensions:
50mm (W) x 72mm (D) x 71mm (H)
Connections:
HD-DB-15-style through connector on module underside
Driver Modules:
Quantity:
2 types, high power and standard power
Max Power Delivery:
75W per driver/12.5V max/14A max
Dimensions:
71mm (W) x 33mm (D) x 65mm (H)
Connections:
DB-25-style through connector on module underside
Dichroic Combiner Modules:
Dimensions:
38mm (W) x 55mm (H) x 3mm (D)
XLED1 System:
Power Consumption:
Supply:
Fuse Rating:
Front Panel Indicators:
Front Panel Switches:
Front Panel Ports:
Rear Panel Ports:
